리시안셔스 (Eustoma grandiflorum)는 세계적으로 널리 재배되는 경제적으로 중요한 원예작물이지만 다양한 병원균에 의한 피해를 입고 있다. 2023년 12월 김해시에서 재배되고 있는 리시안셔스 잎이 어둡게 변색되고 시드는 병징이 발견되었다. 국내 리시안셔스 재배지에서 현재까지 이러한 질병은 보고된 바 없는 것으로 알려졌다. 본 연구에서는 리시안셔스의 병든 잎에서 병원균을 분리하였고, 형태학적, 분자생물학적 동정을 실시하였다. 병원균의 비교적 느리게 성장하며, 형태학적 특성은 균사가 밀집된 형태의 균총과 얇고 긴 형태의 분생포자(39.6 × 2.82 μm)를 형성하였다. 병원균의 분자생물학적 동정을 위해 ITS, LSU 영역 염기서열 분석을 실시하였고, GenBank 데이터와 비교한 결과 병원균은 Pseudocercospora eustomatis로 동정되었다. 추가적으로 리시안셔스에 대한 P. eustomatis의 병원성 검정을 실시한 결과, 포장에서와 동일한 병징이 관찰되었다. 본 논문은 국내에서 P. eustomatis에 의해 발생한 리시안셔스 점무늬병에 대한 최초 보고이다.

This study was carried out to estimate the effect of selected inoculants on chemical compositions and fermentation characteristics of rye silage. Rye was harvested at dough stage and divided into 5 treatments, following: No additives (CON); L. plantarum R48-27 (LP27); L. buchneri R4-26 (LB26); Mixture of LP27 and LB26 at 1:1 ratio (MIX); and L. buchneri (LB). The rye forage was ensiled into 10 L bucket silo for 100 days. The contents of NDF and ADF were lowest (P<0.05) in LB26. The pH in LB26, MIX, and LB were lower (P<0.05) than CON and LP27. Lactate content in LB was higher (P<0.05) than the others, while acetate content in LB26 and LB were higher (P<0.05) than that in CON and LP27. Lactate to acetate ratio was highest (P<0.05) in LB, but lowest in LB26. Lactic acid bacteria (LAB) count in LB was higher (P<0.05) than that in CON, while yeast count in CON was lower than in all silages applied inoculants. In conclusion, silages inoculated with LB26 could improve potentially the aerobic stability caused by increases of acetate and propionate concentrations.

This study was conducted to estimate the effect of home or hetero fermentative lactic acid bacteria(LAB) on chemical composition, fermentation quality, and aerobic stability of rye silage. Rye forage was harvested at dough stage(28.9% of dry matter), chopped to 3-5 cm length, and divided into 4 piles for different inoculations as treatment, following 1) No additives(CON); 2) Lactobacillus plantarum at rate of 1.5 x 105 cfu/g of fresh forage(LP); 3) L. buchneri at rate of 1.2 x 105 cfu/g of fresh forage(LB); and 4) Mixture of LP and LB at 1:1 ratio(MIX). Rye silage was ensiled into 20 L bucket silo in quadruplicate for 0, 1, 4, 7, and 100 day periods. After 100 days of ensiling, the silage treated with LB had lower acid detergent fiber content(p<0.05), but higher in vitro dry matter digestibility(p<0.05). The LB and MIX reduced (p<0.05) pH more rapidly than CON and LP across the ensiling days, but had no difference on 100 days. Silage treated LP had lowest(p<0.05) acetic acid, but highest(p<0.05) propionic acid. In contrast, LB treated silage had highest(p<0.05) acetic acid, but lowest(p<0.05) propionic acid with the absence of butyric acid. On microbial count, LP treated silage had lowest(p<0.05) LAB, yeast, and aerobic stability, whereas LB and MIX treated silages had highest(p<0.05). Mold was not detected across all silages. Therefore, it could be concluded that heterofermentative LAB solely or combo with homofermentative LAB might improve in vitro dry matter digestibility, fermentation characteristics, and aerobic stability of rye silage harvested at dough stage.