From the used sawdust medium of commercial mushroom, Lyophyllum ulmarium and Pleurotus eryngii, cellulase was extracted by distilled water. Optimum temperature of extraction was 25 ℃. Cellulase including this medium could attack cellulose powder, wood tip of a Japan ceder and cypress, and rice hull. Both cellulase from Lyophyllum ulmarium and Pleurotus eryngii showed high activities for rice hull. Now, by affinity chromatography, these cellulase is tried to purify.