The objective of this study was to investigate the methods of quality maintenance using modified atmosphere packaging with various quality preservatives at ambient and low temperatures. Ethylene() caused quality deterioration such as yellowing and abscission. The addition of potassium permanganate reduced ethylene level and thus extended storage life of l00% at 20 and 140% at 0. To establish the storage conditions of minimally processed sit and vegetables, the experiment was conducted with various quality preservatives and packaging materials. Ceramic film(Zeolite 7%, 60) showed increasing storage life of 50%, reducing total microbial counts of 50% and keeping high appearance quality of minimally processed pears stored at 20 and 0. Minimally processed Chinese cabbage treated with l% CaCl2 at 20, and 1% CaCl2 and 1% NaCl at 0 has a longer storage life of 90% with good maketable quality. Treatment of 1% NaCl for cut Asian penis appeared increased storage life of 100%, decreased cut surface browning and the best overall acceptability by a sensory panel

In our studies on the role of -galactosidase in fruit softening, significant difficulty, was encountered in our attempts to extract RNA from persimmon(Diospyros kaki L. cv. Fuyu) fruit due to astringency and tannin content. Initial, unsuccessful RNA extractions involved methods using guanidinium isothiocyanate/CsCl with and without polyvinylpyrrolidone(PVP), phenol/sodium lauryl sulfate(SDS), guanidinium hydrochloride, as well as polysomal RNA purification method that used 0.2 M Tris-HCI (pH 9.0) containing KCI, Mg-acetate, EDTA, -mercaptoethanol, and sucrose. A method was devised which employed treatment of fruit with CO2 gas to diminish astringency prior to RNA extraction, followed by extraction of tissue powders with Proteinase K extraction buffer containing PVP and ascorbate at an alkaline pH. This procedure resulted in the removal of tannins and other polyphenolics and extraction of relatively large amount of high-quality RNA suitable for cDNA library construction and polymerase chain reaction(PCR). Futhermore, the procedure does not use the toxic and corrosive chemical guanidinium isothiocyanate or require ultracentrifugation.

하우스 딸기 재배에 있어서 2, 4-D(아민감 40%)의 업면시용 효과를 알기 위하여 1 화방의 1번과 개화기(2월 11 일)에 10ppm 농도로 10 a당 120 를 처리하였던 결과, 1. 딸기는 엽병장 및 과경장이 길어지면서 상편생장을 하였고 잎과 과실로부터 에틸렌 생성이 증가되었다. 2 딸기는 에틸렌 생성 증가에 따른 수확최감기가 15일 정도 촉진되었고 과실비대로 1 과중이 증가되어 18 %의 증수 효과를 가져왔다.