다환 방향족 탄화수소(PAHs)에 의한 오염이 미생물군집구조에 미치는 영향을 파악하기 위하여 연안에 가까운 광양만 퇴적토를 대상으로 하여 2000년 3월과 8월 2회에 걸쳐 PAHs 오염도와 말단제한절편 다형성 (T-RFLP)분석방법을 이용하여 미생물 군집구조를 조사하였다. T-RFLP방법으로 조사한 미생물 군집은 계절에 따라 군집이 구분되었으며 월내천입구에 위치한 정점 1에서 3월에 다른 정점과 다른 독특한 군집구조를 형성하였다. 또한 상대적으로 PA

Anaerobic digestion is one of the most economical ways to treat organic wastes; the production of excessive sludge is limited; aeration is not required; operation cost is low; valuable energy resource (methane) is produced. However, propionate, an intermediate substance during the process, is known to inhibit biochemical process at high concentration; but it is difficult to be degraded biologically as its oxidation is thermodynamically unfavorable (△G>0). However, there are several microorganisms which could oxidize propionate fully by their syntrophic association. Therefore, in this research, the propionate enrichment culture was produced in large amount (~1L), and the main players which are responsible for utilizing propionate are identified. Furthermore, the kinetic parameter was obtained from this research. Seed came from two different full-scale anaerobic digesters which is located in Joongrang (JR) and Sincheon (SC). 16s rRNA gnee based metagenomics results indicated that Smithella propionica and Methanobacterium beijingense were the most dominant species in all seeds. The fact that bacterial family Syntrophomonadaceae and archaeal genus Methanosaeta were also found significantly in all seeds means that there might be symbiotic relationship among propionate oxidizing bacteria, butyrate oxidizing bacteria, hydrogenotrophic methanogens and acetotrophic methanogens in complete oxidation of propionate. Based on the first order kinetic which indicated propionate degrading rate, k (1/day) on JR and SC was represented 0.0243 and 0.0223, respectively. Also, based on Modified-Gompertz model, umax on JR and SC was represented as 0.024 and 0.016, respectively.