This study presents design, construction and testing of a immiscible two working fluids thermosyphon with water and FC-40 to prevent freezing of vehicle starting up at temperatures below freezing point of water. The two working fluids thermosyphon was experimentally investigated on the startup characteristics at low temperatures below 0°C and then the thermosyphon was stable during startup and operated reliable at temperature of –30°C. The testing also showed that the performance and characteristics of the two working fluids thermosyphon differ from the charging ratio and mixture ratio of the two working fluids.