본 연구에서는, F4-ZnPc 광활성층 기반의 저분자 유기태양전지의 성능을 최적화 하기 위해서 다 양한 조건의 홀이동층과 전자이동층을 조합하는 연구를 진행하였다. BF-DPB 호스트 유기물에 C60F36 또 는 NDP9 도펀트를 도핑한 조합을 홀이동층으로 사용하였고, 전자이동층으로는 W2(hpp)4가 도핑된 C60 또는 순수 C60/Bphen 물질을 사용하였다. 다양한 홀/전자이동층의 조합은 유기태양전지의 단락전류밀도와 fill factor, 효율에 영향을 끼치는 것을 관찰할 수 있었다.
This study was undertaken to optimize enucleation and reconstitution methods for the production of cloned mice by somatic cell nuclear transfer Outbred ICR mouse oocytes at the metapahse- II stage were retrieved from female mice superovulated by PMSG and hCG. In Experiment 1, oocytes were enucleated in medium supplemented with cytochalasin B (CCB) of 3 levels (0, 7.5 or 15 /mL), and higher rate of encleation was obtained at 7.5 and 15 /mL than at /mL. In Experiment 2, oocytes enucleated in 7.5 /mL CCB-containing medium were reconstituted with different types of somatic cell by following methods; 1) cumulus cells by direct cell injection, 2) cumulus cells by electric fusion (1.25 kV/cm, 2 pulses for each 70 ) or 3) STO cells by the electrofusion. Electrofusion of STO cells with enucleated oocytes yielded the greatest (P<0.05) rate of reconstitution without lysis (76%) than any other combinations. Although significant decrease in the rate of somatic cell introduction was found, the electrofusion of cumulus cells yielded better rate of reconstitution than direct injection (0 vs. 18%). In Experiment 3, the duration of electric stimulation for the fusion was changed to either 50 or 90 , but no significant improvement of reconstitution efficacy was obtained. In conclusion, this study showed that ICR mouse oocytes could be used for the production of reconstituted oocytes and a fusion method of 1.25 KV/cm with 2 pulses using 570 cell was the optimal.