This study aimed to investigate the psychological effect of a healing agricultural program using insect resources and to confirm the persistence of the healing effect. The applied insect was silkworm Bombyx mori (Linnaeus) and 28 adolescents from local children’s centers were used as the subjects. The healing agricultural program was applied at the centers for 4 weeks, and stress was measured using salivary ɑ-amylase levels and a survey on life satisfaction, happiness, and insect preference. The results indicated that stress levels decreased, and life satisfaction and insect preference increased after participating in the the healing agricultural program. Furthermore, it was confirmed that this healing effect continued until 4 weeks after the end of the program.