Indoor air quality can be affected by indoor sources, ventilation, decay and outdoor levels. Although technologies exist to measure these factors, direct measurements are often difficult. The purpose of this study was to develop an alternative method to characterize indoor environmental factors by multiple indoor and outdoor measurements. Using a mass balance model and regression analysis, penetration factor (ventilation rate divided by the sum of ventilation rate and deposition constant) and source strength factor (source strength divided by the sum of ventilation rate and deposition constant) were calculated using multiple indoor and outdoor measurements. Subsequently, the ventilation rate and NO2 generation rate were estimated. Mean of ventilation rate was 1.41 ACH in houses, assuming a residential NO2 deposition constant of 0.94 hr-1. Mean generation rate of NO2 was 16.5 ppbv/hr. According to house characterization, inside smoking and family number were higher NO2 generation rates, and apartment was higher than single-family house. In conclusion, indoor environmental factors were effectively characterized by this method using multiple indoor and outdoor measurements.