Since airborne fungi have been known to aggravate indoor air quality, studies on developing anti-fungal filters increase recently. In this study, silver (Ag) nanoparticle was selected as anti-fungal agent. HEPA filter was coated with silver nanoparticles which were generated via spark discharge system operating at atmospheric pressure and temperature. The anti-fungal effect of the Ag-filter was evaluated with the conventional culture assay. When the number of Ag nano particle per a fungal particle in the filter was 1.91X106, the fungicidal efficiency was higher than 99%. As another anti-fungal test, ATP bioluminiscence detection method was also carried out and the results were correlated with those of the culture assay.