Laparoscopic appendectomy using three trocars has been widely performed. Recently, a single incision laparoscopic surgical procedure has become popular because it is less invasive. We report on our early experience in performance of single incision laparoscopic appendectomy (SIL-A). Data on the operating time, the duration to make the pneumoperitoneum, body mass index (BMI), rate of conversion to another operation, complication, hospital stay, dosage of analgesics, and pain score were collected prospectively at two centers. Twenty seven patients underwent SIL-A. The mean BMI was 23.3±3.1 kg/m2 (17.6-30.5). The mean time required to make a pneumoperitoneum was 9.9±5.0 minutes (5.0-28.0). The mean operating time was 37.6±26.7 minutes (10.0-100.0). The rate of intra-operative complication was 3.7% (1/27), and the rate of post-operative complication was 22.2% (6/27). The mean hospital stay was 4.1±1.2 days (2-6). The immediately postoperative pain score was 5.4±1.5 (3-8), however, the pain score at discharge was 0.4±0.7 (0-2). The mean dosage of analgesics was 1.5±1.3(0-4), however, none of the patients took analgesics after postoperative three days. SIL-A is feasible but requires improved instrumentation and experience.