As the size of the wind turbine tower becomes larger and larger, research on assembled wind turbine tower that is advantageous for transportation and installation is continuing. Large wind turbine tower require door openings for maintenance. The opening of the tower has an irregular cross section, and an excessive stress is generated due to the door opening. The result is structural damage to the tower and many accidents. In this research, stress analysis was performed on a model with internal stiffener to prevent excessive stress. The stress was investigated around the openings where the tower was resonant and excessive stressed, and the shape of the openings was optimized. Through optimization, we confirmed that the maximum stress was reduced by about 6% with respect to the initial value.