This study aimed to measure the in-situ target strength (TS) of the moon jellyfish (Aurelia aurita) using a 200 kHz scientific echo sounder in a natural coastal environment. The acoustic survey was conducted in the coastal waters of Soho Port, Yeosu, Korea, from June 4 to 5, 2024. TS measurements were performed by installing the transducer in both horizontal and vertical orientations, and the TS distribution characteristics were analyzed. The measured TS values ranged from -89.8 to -64.8 dB in the horizontal direction and from -90.0 to -59.1 dB in the vertical direction, showing no significant difference between detection orientations (p<0.05). Additionally, a bell diameter-wet weight relationship for Aurelia coerulea was derived based on the specimens collected at the survey site. The empirical TS model proposed by Mutlu (1996) was applied to estimate TS values using the measured morphological data, and the results exhibited a similar trend to the field-measured TS distribution. These findings provide fundamental data for acoustic monitoring and stock assessment of jellyfish populations in natural environments.