This study evaluated the virucidal efficacy of a Bacillus complex composed of Bacillus subtilis YBK-70, Bacillus atrophaeus YBK-W4, and Bacillus circulans YBK-04 (1:1:1, v/v/v) against porcine epidemic diarrhea virus (PEDV). Virucidal efficacy was assessed by measuring the infectivity of PEDV in Vero cells after exposure of the virus to the Bacillus complex culture medium (BCCM) (4×10⁶ colony-forming units (CFU)/mL). PEDV was exposed to the BCCM in the presence of either hard water (HW) or an organic matter suspension (OM). Under HW conditions, PEDV was inactivated by a 50-fold dilution of the BCCM. In the presence of OM, the disinfectant exhibited virucidal activity at a 10-fold dilution. Because BCCM possesses virucidal activity against PEDV, it may serve as an effective reagent for limiting the spread of animal viral diseases.