Bemisia tabaci is a species complex consist of about 40 cryptic species. This species is also a vector of at least 100 begomovirus including Tomato yellow leaf curl virus (TYLCV). TYLCV transmitted by B. tabaci in a circulative propagative manner but its molecular mechanism is remain unclear. We investigate the transcriptional response of B. tabaci MED to TYLCV infection using next generation sequencing (NGS) analysis. In total 16757 differentially expressed genes between viruliferous and non-viruliferous whiteflies were analyzed. Among them 289 transcripts found significant, where 116 up-regulated and 173 down-regulated. Our results reveal the whitefly-TYLCV relationship and provide important molecular information about virus transmission of vector insects.

The Bemisia tabaci (Hemiptera: Aleyrodidae) is a pest that sucks a plant's phloem, excretes nectar and infects tomatoes with TYLCV (Tomato Yellow Leaf Curl Virus), causing severe economic damage. In order to explore the cause for occurrence of areas where the genetic structure of B. tabaci MED (Mediterranean) was reversed in a short period of time, this study was conducted. We collected and analyzed the B. tabaci MED populations from tomato greenhouses in some areas where their genetic structure was reversed between two years 2016 and 2017. As a result, the genetic structure of B. tabaci MED populations was found to be close between populations of the nursery and the greenhouses where tomato seedlings were supplied from that nursery, even if greenhouses were located far away each other. Further study is needed to identify the causes for occurrence of abrupt reverse of genetic structures of B. tabaci MED populations observed in some areas throughout the country.