The fruit of Chaenomeles sinensis (Thouin) Koehne (Chaenomelis Fructus) known as “Mo-Gua” in Korea has been commonly used in traditional medicine to treat inflammatory diseases, such as sore throat. However, its effect on bone metabolism has not been elucidated yet. Here, we examined the effect of Chaenomelis Fructus ethanol extract (CFE) on receptor activator of nuclear factor (NF)-κB ligand (RANKL)-mediated osteoclast differentiation and formation. CF-E considerably inhibited osteoclast differentiation and tartrate-resistant acid phosphatase-positive multinuclear cell formation from bone marrow-derived macrophages and osteoclast precursor cells in a dose-dependent manner. In addition, the formation of actin rings and resorption pits were significantly suppressed in CF-E-treated osteoclasts as compared with the findings in non-treated control cells. Consistent with these phenotypic inhibitory results, the expressions of osteoclast differentiation marker genes (Acp5, Atp6v0d2 , Oscar, CtsK, and Tm7sf4) and Nfatc1 , a pivotal transcription factor for osteoclastogenesis, were markedly decreased by CF-E treatment. The inhibitory effect of CF-E on RANKL-induced osteoclastogenesis was associated with the suppression of NFATc1 expression, not by regulation of mitogen-activated protein kinases and NF-κB activation but by the inactivation of phospholipase C gamma 1 and 2. These results indicate that CF-E has an inhibitory effect on osteoclast differentiation and formation, and they suggest the possibility of CF-E as a traditional therapeutic agent against bone-resorptive diseases, such as osteoporosis, rheumatoid arthritis, and periodontitis.

모과의 이용 가능성을 검토하기 1, 3 및 5% 모과 물 추출액을 담금용수로 물김치를 제조하여 발효특성을 조사한 결과 발효 초기 pH는 대조구 6.53, 1, 3 및 5% 모과 물김치의 pH는 각각 4.14, 3.61, 3.54이었으며, 발효과정 중 대조구는 감소하였으나, 모과 물김치의 변화는 완만하였다. 물김치의 산도 변화는 pH 변화와 유사하였다. 물김치 발효 중 총 균수와 젖산균수의 변화는 유사하였으며, 모과 물김치의 발효과정 중 젖산균수는

본 연구에서는 in vitro에서 모과의 항산화 활성을 평가하기 위하여 용매별 분획물의 항산화능을 검토하였다. 모과에탄올 추출물의 수율은 9.23%이었고, 이를 n-hexane, chloroform, ethylacetate, n-butanol, water로 계통 분획한 수율은 water분획물이 1.52%로 가장 높았으며 그 다음으로 ethylacetate 분획물 1.46%의 순이었다. 모과 에탄을 추출물의 총 flavonoid 함량은 이었고, 각