Cicinela chinensis (Coleoptera : Cicindelidae) is usually distributed from Asian countries; Korea, Vietnam, China and Japan. In larval stage, they make hole on ground and eat small insects that pass over the hole, adult also predator on other small insects and they can move fast. They have regional morphological variation, usually variations were showed in elytral pattern and body colors. There are four subspecies, Cicindela chinensis chinensis, Cicindela chinensis flammifera, Cicindela chinensis japonica and Cicindela chinensis okinawana in Cicindela chinensis all of the world. Because of a lot of variation in elytra pattern and body color, identification of Cicindela chinensis is difficult. Therefore to know the distinct differences, comparison analysis by DNA sequence in COI gene was examined. In this paper COI comparison analysis of Cicindela chienesis from different location was examined. Also COI comparison analysis of species with different elytra pattern and body color collected in same location was done. Although they have different color pattern is same region, the result of COI sequence was same. Otherwise, although they have same color pattern in other regions, the result of COI sequence was different.