The impact of transgenic Bt maize plant contained Cry1F was evaluated on the oat aphid Rhopalosiphum padi as a non-target insect species. Slightly reduced rates of survival and alata vivipar production were observed on Bt maize than on the non-Bt maize. In addition, slightly low preference to Bt maize plant was observed. Aphid fecundity, measured as the number of offspring produced for 7 days, was higher on Bt maize than on non-Bt maize but not different significantly. ELISA test using Cry1F-antibody revealed that 26% of Cry1F protein compared to the positive control was detected from the whole body of R. padi when the insects were fed Bt maize for 50 days, showing that R. padi can carry Cry1F protein to the higher trophic level when exposed to Bt maize. Taken together, the Bt maize plant is not likely to cause any negative side impacts on non-target insect R. padi but Bt toxin can be transferred to higher predators via R. padi as it carries the toxin.