This research modified the checklist used in the universities in USA, England and Korea considering the current situation of Korea, and conducted case studies upon each section of appointed chemical laboratories based on 4M criteria, which stands for Machine, Media, Man and Management. The purpose of the studies is to assess how dangerous the laboratories are and to contribute to prevention of accidentsin the laboratories as well as reducing loss of lives and property. The result of this research found out the harmful and hazardous factors based on the 4M-type checklist and predicted the dangerousness as it multiplies possible frequency by intensity. Protective equipment, safety facilities in laboratories, emergency exit and compressed gas cylinder are found to be more dangerous, so the result shows that it is desirable to improve ventilation, safety facilities and circumstances of the laboratory through the investment.