To investigate seasonal variation and species composition by depth layers in the deep sea ecosystem of the East Sea of Korea, bottom trawl survey was conducted at 4 depth layers during spring and autumn from 2007 to 2009. A total of 47 species were collected and were composed of 23 fish species, 9 crustacea, 6 cephalopoda and 9 gastropoda. The main dominant species at each depth layers were Chionoecetes opilio in 300m, Berryteuthis magister in 500m, Chionoecetes japonicus in 700m and 900m. In spring, richness indices (R) showed low value of 2.01 in 500m depth, and high value of 2.16 in 300m depth. Diversity indices (H') showed low value of 1.53 in 300m depth, and high value of 2.09 in 700m depth. Dominance indices (D) showed low value of 0.15 in 700m depth, and high value of 0.31 in 300m depth. In Autumn, richness indices showed low value of 1.48 in 900m depth, and high value of 2.69 in 300m depth. Diversity' indices (H') showed low value of 1.13 in 300m depth, and high value of 2.23 in 700m depth. Dominance indices (D) showed low value of 0.14 in 700m depth, and high value of 0.54 in 300m depth. In spring, similarity analysis in each depth layers showed the difference between 900m and othe depth layer, on the contrary 500m and 700m showed the similarity. In autumn, similarity analyssis in each depth layers showed the difference between 700m and other depth layers, on the contrary 300m and 500m showed the similarity.