The purpose of this study was to examined the antioxidant activities by water and 70% ethanol extract from durian (Durio zibethinus.) seed, sarcocarp and peel. Durian extract were studied for reducing sugar content, polyphenol content, superoxide dismutase (SOD) like activity, electron donating ability, nitrite scavenging ability, flavonoid content, hydroxy radical scavenging activity. Reducing sugar content were increased peel 〉 sarcocarp 〉 seed. Total polyphenol, flavonoid content, DPPH radical scavenging ability and SOD like activity were increased seed 〉 peel 〉 sarcocarp. Total polyphenol content was relatively high as 21.90±0.50mg/g in the ethanol extract of the seed. DPPH radical scavenging ability was relatively high as 62.08±2.63% in the water extract of the seed. Nitrite scavenging ability was no significant difference. Hydroxy radical scavenging activity was increased seed 〉 peel 〉 sarcocarp, was relatively high as 58.27±1.13% in the water extract of the seed.