간행물

한국약용작물학회지 KCI 등재 Korean Journal of Medical Crop Science

권호리스트/논문검색
이 간행물 논문 검색

권호

Vol.21 No.4 (2013년 8월) 9

1.
2013.08 서비스 종료(열람 제한)
Collected germplasms of five representative dandelion species (Taraxacum ohwianum, T. platycarpum, T. platypecidum, T. officinale, and T. coreanum) were 104 lines from different habitates in Korea and China. Their genetic diversity was analyzed by genomic fingerprinting method using amplified fragment length polymorphism (AFLP). AFLP results of 6 primer combinations were revealed 1,176 total DNA fragments and 523 polymorphic bands with a 44.4% ratio of polymorphism. On the basis of similarity coefficient analysis by unweight pair group method with arithmetic averages (UPGMA), 104 dandelion germplasm lines were ranged from 0.64 to 0.99 and clustered distinct five group depending on the species. Furthermore, a principal coordinate analysis (PCA) by the application of multi-variate analysis indicated significantly greater differences among species than geographical origins.
2.
2013.08 서비스 종료(열람 제한)
The purpose of this study was to examined the antioxidant activities by water and 70% ethanol extract from durian (Durio zibethinus.) seed, sarcocarp and peel. Durian extract were studied for reducing sugar content, polyphenol content, superoxide dismutase (SOD) like activity, electron donating ability, nitrite scavenging ability, flavonoid content, hydroxy radical scavenging activity. Reducing sugar content were increased peel 〉 sarcocarp 〉 seed. Total polyphenol, flavonoid content, DPPH radical scavenging ability and SOD like activity were increased seed 〉 peel 〉 sarcocarp. Total polyphenol content was relatively high as 21.90±0.50mg/g in the ethanol extract of the seed. DPPH radical scavenging ability was relatively high as 62.08±2.63% in the water extract of the seed. Nitrite scavenging ability was no significant difference. Hydroxy radical scavenging activity was increased seed 〉 peel 〉 sarcocarp, was relatively high as 58.27±1.13% in the water extract of the seed.
3.
2013.08 서비스 종료(열람 제한)
The purpose of this study was to research for anti-oxidation and anti-wrinkle effects of Acar mono Sap (AM). To cosmetic effect of AM, safety effect (MTT assay), anti-wrinkle effect (elastase, MMP-1 inhibition assay) and anti-oxidant effect (DPPH assay) were measured. When water extract of AM was used for cell viability, it was over 100% at 6% (6 ml/100 ml in phosphate buffer) concentration. AM showed 45.7% elastase inhibition and 23.7% MMP-1 inhibition at 50% (50 ml/100 ml in phosphate buffer) concentration so that it had good anti-wrinkle characteristic. And AM showed 68.9% antioxidation capacity at 50% concentration by using a DPPH assay. Consequently, AM can be used as natural materials or additives for human skin owing to their beneficial biologic functions, including the anti-wrinkle effect, for cosmetic compositions.
4.
2013.08 서비스 종료(열람 제한)
This text was analyzed and investigated the distribution of medicinal plants in Cheongoksan Bonghwagun Korea, in order to search the medicinal resources that are used in modern medicine. Medicinal plants of the Korean Pharmacopoeia (10th edition) distributed in Cheongoksan Bonghwagun were consisted of 93 taxa ; 82 species, 10 varieties, 1 forma of 79 genus, 50 families. In medicinal plants of the Korean Pharmacopoeia, rate of native species and exotic species was 89.2% (83 taxa) and 10.8% (10 taxa) respectively. Family classification was the most of compositae of 8 taxa, and life form classification was most of herb of hemicryptophyte species. The classification by using parts were 34 taxa of root use and the classification of efficacy utilization was 24 taxa of Cheongyeolyak (heat-clearing drug) use.
5.
2013.08 서비스 종료(열람 제한)
This study was conducted to compare the contents of ginsenoside according the water extract conditions of red ginseng. In method A, red ginseng extract was prepared at 75℃ for 18 hours by 1 time extraction, and method B, the preparation was done at 85℃ for 18 hours by 1 time extraction. In method C, the primary extract prepared at 75℃ for 9 hours was blended with the secondary extract prepared by re-extracting the red ginseng residue obtained after the primary extraction, at 85℃ for 9 hours. Method D was the same procedure as method C but the extraction temperature for the primary extraction was 85℃ and that for the secondary extraction was 95℃. The contents of total and Rb1, Rg1 and Rg3 ginsenoside were highest in Method C. The content of prosapogenin (ginsenoside Rg2, Rg3, Rb1 and Rb2) was highest in Method B. There was no consistent tendency in Brix, pH, Hue value and absorbance among extraction methods.
6.
2013.08 서비스 종료(열람 제한)
Gastrodia elata Blume is a achlorophyllous orchid plant that has been used in traditional medicine to treat many human illnesses, such as vertigo, hemiplegia, and epilepsy. It contains three major pharmacological substances; gastrodin, ergothioneine, and vanillyl alcohol. Here we investigated the quantitative changes in those functional components by cultivation conditions and harvest times. The content of ergothioneine was different with cultivation areas, while gastrodin accumulated the higher levels than vanillyl alcohol regardless of cultivation areas. The smaller tubers contained the higher concentrations of gastrodin and the lower concentrations of ergothioneine than did the larger tubers. The cultivation under sun shade screen increased the concentrations of both gastrodin and vanillyl alcohol but decreased the levels of ergothioneine. We also determined the contents of functional substances depending on the harvest times. The concentration of gastrodin was higher in October than in April, while the level of ergothioneine was higher in April than in October. Interestingly, the use of sexually propagated seed tubers produced 1.9 times higher levels of gastrodin compared to vegetatively propagated seed tubers. In this study, we found that three major functional substances in G. elata tubers altered depending on various cultivation practices, which might be important to increase the economic values of G. elata tubers.
7.
2013.08 서비스 종료(열람 제한)
This study was carried out to investigate the cause of leaf discoloration occurring frequently in paddy cultivation. Chemical property of soil and inorganic nutrient component of leaf were analyzed on abnormal fields of 7 regions where leaf discoloration occurred severely and normal fields of 7 regions among ginseng garden. The pH of abnormal fields was strong acidic condition (pH 5.51) compare to normal fields of slightly acid condition (pH 6.42). Calcium and magnesium content in abnormal fields were lower distinctly than that of normal fields, while EC, organic matter, phosphate, and potassium content showed not distinct difference between abnormal and normal fields. Whereas calcium and magnesium content were distinctly high in normal fields, both of potassium and iron content of ginseng leaf were distinctly high in abnormal fields. In particular, iron content of abnormal fields was more 1.94 times in soil, and 3.03 times in leaf than that of normal fields. In soil chemical property, there were significant negative correlation between leaf discoloration ratio and soil pH, and there were also significant positive correlation between leaf discoloration ratio and iron content. In ginseng leaf, there were highly significant negative correlation between leaf discoloration ratio and calcium content, and there were also highly significant positive correlation between leaf discoloration ratio and iron content.
8.
2013.08 서비스 종료(열람 제한)
This study was carried out to have the basic and applied information to develop the cultivation methods and models and to increase the productivity of high-quality ginseng. Plant height was dramatically increased from one year old to four years old, and then it was grown up very slowly. Stem length was shown similar tendency as the plant height did. Its range were from 9.4 cm in one year old to about 42.4 cm in four year-old ginseng. The leaf area was clearly increased until four years old, 10.1cm2 in one year old to 204.9cm2 in four years old, while it was slightly increased after four years old. Root length was continuously increased from one year old until four years old, and then it was grown up very slowly. Tap root length which was measured was appeared about 8 cm from three years old to six years old, showing not much different among the year-olds. The root diameter was continuously increased from one year old to six year-old ginseng, about 2.9 mm and about 19 mm, respectively. The moisture content was higher on the shoot than that on the root. It was slightly decreased as they were getting old. In shoot part, the rate of leaves and stems based on dry weight was about 1 : 1. In the root, the dry weight of tap root was heavier than that of lateral root. The dry weight of shoot was decreased after four years old, while it of the root was continuously increased until six years old, resulting the production of ginseng root. Based on the results of this study, growth characteristics of shoot and root of ginseng were dramatically increased from one year old to four years old and then their growths were appeared different between shoot and root, showing not much growing in shoot and keep growing in root.
9.
2013.08 서비스 종료(열람 제한)
This study was performed to select adequate plant materials for developing a natural α-glucosidase inhibitor by analyzing α-glucosidase inhibition activity in fronds and rhizomes of three Dryopteridaceae species: Cyrtomium fortunei, Polystichum polyblepharum, and P. lepidocaulon. The highest α-glucosidase inhibitor obtained from frond of P. lepidocaulon (4.16μg·mL-1), and rhizome of C. fortunei (1.84μg·mL-1), showed much higher inhibition activity than acarbose (1413.70μg·mL-1). The biomass required to inhibit α-glucosidase by 50% was 0.04 ~ 0.35mg for frond and 0.03 ~ 0.10mg for rhizome, and P. lepidocaulon required the least amount of fronds and P. lepidocaulon the least rhizomes. In frond, α-glucosidase inhibition activity was the highest in water fraction of C. fortunei (20.2μg·mL-1), and n-butanol fraction of P. lepidocaulon (9.33μg·mL-1) and P. polyblepharum (5.10μg·mL-1). In rhizome, it was the highest in n-butanol fractions of C. fortunei (19.76μg·mL-1) and P. polyblepharum (4.47μg·mL-1), and ethylacetate fraction of P. lepidocaulon (5.46μg·mL-1). The frond biomass required for 50% α-glucosidase inhibition was the lowest in the water fraction of C. fortunei (1.43mg), and n-butanol fractions of P. lepidocaulon (1.10mg) and P. polyblepharum (0.66mg). The required biomass of rhizome was the lowest in the water fraction of C. fortunei (1.59mg), and n-hexane fractions of P. lepidocaulon (0.04mg) and P. polyblepharum (0.15mg). The result of this study suggested that the three Dryopteridaceae species had high α-glucosidase inhibition activity with small biomass, which might have high value as materials for economical anti-diabetic medication.