간행물
한국약용작물학회지 KCI 등재 Korean Journal of Medical Crop Science
- 발행기관 한국약용작물학회
- 자료유형 학술지
- 간기 격월간
- ISSN 1225-9306 (Print)2288-0186 (Online)
- 수록기간 1993 ~ 2019
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 524DDC 633
권호리스트/논문검색
Vol.22 No.1 (2014년 2월) 8건
1.
2014.02
서비스 종료(열람 제한)
Codonopsis lanceolata (Campanulaceae) has been used in traditional medicines, as its roots contain severalkinds of 3,28-bidesmosidic triterpenoid saponin with high medicinal values. In this study, we induced hairy root-derivedtransgenic plants of C. lanceolata and analyzed triterpenoid saponins from the leaf, stem and root. Transgenic plants wereregenerated from the hairy roots via somatic embryogenesis. The saponins are lancemaside A, B and E, foetidissimoside A,and aster saponin Hb. Transgenic plants contained richer triterpenoids saponin than wild-type plants. Major saponin lance-maside A was the most abundant saponin in the stem from transgenic-plant, 4.76㎎·1−¹ dry stem. These results suggest thattransgenic plants of C. lanceolata could be used as medicinal materials for the production of triterpene saponins.
2.
2014.02
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The present study was carried out to evaluate the antioxidant and anti-inflammatory activities of threeextracts (hot water, 50% ethanol and mixed solvent;water, ethanol, butylene glycol, propylene glycol) of dried chestnut,chestnut shell, chestnut leaves and dried chestnut leaves obtained from Castanea crenata tree. When conducted DPPH assay,radical scavenging activity of ethanol extract of chestnut shell was the highest with IC50 10.8㎍/mL among four extractsfrom these parts (p<0.05). In additional results by the xanthine oxidase assay, antioxidant activity showed that waterextract of chestnut leaves showed the highest xanthine oxidase inhibitory activity in the tested extracts (p<0.05). Futher-more, extracts of chestnut shell and leaves exhibited no cytotoxicity in RAW 264.7 cells (p<0.05). Also, anti-inflammatoryactivity by NO assay showed LPS-induced NO was significantly inhibited following treatment with extracts of chestnut shelland leaves of 3㎎/mL (p<0.05). These data suggest that extract of chestnut shell have antioxidant and anti-inflamantoryactivity including chestnut leaves. Therefore, it is considered that Castanea crenata research range and selection of functionalmaterial can broaden chestnut shell to other fractions such as chestnut and chestnut leaves.
3.
2014.02
서비스 종료(열람 제한)
Comparative Analysis of Metabolites in Roots of Panax ginseng Obtained from Different Sowing Methods
Ginsenosides of roots in Panax ginseng were analyzed by metabolic-targeting HPLC using the partial leastsquares discriminant analysis (PLS-DA) and compared depending on sowing methods between direct seeding andtransplanting method. Score plots derived from PLS-DA could identify the sowing method between the direct seeding andtransplanting method in P. ginseng roots. The ginsenoside compounds were assigned as Rg1, Re, Rf, Rg2, Rb1, Rc,Rb2, Rb3, and Rd. Contents of Re, Rf, Rg2, Rb1, Rc, Rb3, and Rd of main roots produced from the transplanting methodwere relatively higher than those of samples produced from direct seeding method. Also, contents of Rg1, Re, Rf, Rg2, Rb1,Rc, Rb2, Rb3, and Rd of lateral roots from the transplanted samples were relatively higher than those of samples producedfrom direct seeding method. Therefore, HPLC with PLS-DA analysis can be a straightforward tool for identificationof ginsenosides in main or lateral roots of P. ginseng obtained from two different seeding methods between directand transplanting methods.
4.
2014.02
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This study was carried out to investigate change of ginsenoside contents in red and fresh ginseng according toroot part and age by hydrolysis. Neutral total ginsenoside contents by hydrolysis in 6-year main root and lateralroot were significantly increased than those by non-hydrolysis, as 41.6 and 32.8%, respectively. However, there wasno significant difference in red ginseng. In fresh ginseng, ginsenoside contents of the protopanaxatriol group such as Re, Rf,Rg₁, Rg₂, and Rh₁ were not significantly different, but Rb₁, Rb₂, Rb₃, Rc, and Rd showed significant difference. The increaserate of neutral total ginsenoside content by hydrolysis was higher in epidermis-cortex than stele. Also, the neutraltotal ginsenoside content was fine root>rhizome>lateral root>main root, respectively. While there was no tendencytowards the increase of ginsenoside by hydrolysis with the increase of root age in fine root and rhizome, there was significantdecrease in main root and lateral root.
5.
2014.02
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To study the cause of physiological disorder in leaf of ginseng cultivated at paddy soil, the degree of brown-colored symptom (BCS) and the contents of inorganic matter in leaf were investigated by irrigating the solution of ferric andferrous iron of 0.1~2.0%, and citric acid of 1.0~4.0% on bed soil, respectively. Ratio of BCS by variety was as high as85.0% in Yoenpoong, while it was as low as 5.4%, 7.5% in Chunpoong and Hwangsook, respectively. The contents ofinorganic matter of leaf in Yoenpoong were lower in P₂O5, Ca, and Mg, while it were higher in K, Fe, and Mn than othervariety. Iron solution caused BCS more distinctly when each ferric and ferrous iron were dissolved with 1.0% citric acidthan when each iron was dissolved without citric acid. Ferric iron caused BCS more effectively than ferrous iron. BCSoccurred in 4.0% citric acid was as same as 2.0% ferric iron mixed with 1.0% citric acid. Low P₂O5 and high Fe content inleaf appeared in both of artificial and natural symptoms. We concluded that excessive Fe uptake caused BCS to leaf becausethe solubility of iron was increased in condition of low soil pH.
6.
2014.02
서비스 종료(열람 제한)
Effects of Drought Stress on Photosynthetic Capacity and Photosystem II Activity in Oplopanax elatus
This study was performed to investigate the physiological responses of Oplopanax elatus by water condition.Drought stress was induced by withholding water for 26 days. The results show that PN max, SPAD, gs, E and Ci weresignificantly decreased with decreasing of soil moisture contents. However, AQY and WUE were decreased slightly only at26 day. This implies that photosynthetic rate is reduced due to an inability to regulate water and CO₂exchange through thestomatal. According to JIP analysis, ΦPO, ΨO, ΦEO and PIABS were dramatically decreased at 21 day and 26 day, whichreflects the relative reduction state of the photosystem II. On the other hand, the relative activities per reaction center suchas ABS/RC, TRo/RC were significantly increased at 26 day. Particularly, Dio/RC and DIo/CS increased substantially underdrought stress, indicating that excessive energy was consumed by heat dissipation. These results of chlorophyll a fluores-cence show that the sensitivity changes photosystem II activity. Thus, according to the results, O. elatus was exhibited astrong reduction of photosynthetic activity to approximately 10% soil moisture contents, and JIP parameters could be usefulindicator to monitor the physiological states of O. elatus under drought stress.
7.
2014.02
서비스 종료(열람 제한)
In traditional Korean and Chinese medicine, safflower (Carthamus tinctorius L.) for the treatment of centralnervous system-related symptoms such as tremor, seizure, stroke and epilepsy. We investigated the effects of safflower couldinfluence cerebral ischemia-induced neuronal and cognitive impairments. Administration of safflower for 1 day(200㎎/㎏ body weight, p.o.) increased the survival of hippocampal CA1 pyramidal neurons after transient global brainischemia. And neurological functions measured as short term memory. Post-treatment with safflower for 2 times decreasedthe induction/reduction - induced production of neuronal cell loss from global cerebral ischemia. Safflower markedlydecreased neuronal cell death and also caused a decrease in the content of thiobarbituric acid-reacting substances(TBARS) (55.2±9.4µmol mg-¹) and significant improvement of activities of glutathione (GSH) (27.2±5.0µmol mg-¹) inhippocampus. We conclude that treatment with safflower attenuated learning and memory deficits, and neuronal cell lossinduced by global cerebral ischemia. These results suggest that safflower may be a potential candidate for the treatment ofvascular dementia.
8.
2014.02
서비스 종료(열람 제한)
This study was conducted to determine the effects of seed storage temperature and pre-treatment on seed ger-mination, seedling quality and vigor index of wild Trichosanthes kirilowii and Trichosanthes kirilowii var. japonica. As a resultthe highest germination rate of T. kirilowii was the 95% at seed stored in room temperature and then soaking for 24 hours indH₂O. And the highest germination rate of T. kirilowii var. japonica was 96% at seed stored in 2℃ and then soaking for 24hours in GA₃(100ppm). But the seed germination rate was non-significance in pre-treatment at seed stored in roomtemperature 2℃ .Seedlings of T. kirilowii and T. kirilowii var. japonica showed not only the best seedling quality but alsoseedling vigor index in seed stored at 2℃ and then soaking for 24 hours in GA₃(100ppm). In this research, T. kirilowii and T.kirilowii var. japonica seed were stored in room temperature or 2℃, and then sown in peat moss, seed germination rate wasmore than 90% and production of superior quality seedlings.
