본 연구에서는 시설재배와 토경(매립) 복령의 성분 분석 및 생리활성 비교를 위해 연구하였다. 일반성분 분석 결과 산소가 45~46%로 가장 많이 포함하고 있었으며 다음으로 39~41%의 탄소, 6.06~6.1% 수소, 0.21~0.22% 질소로 나타났다. 그리고 시험을 실시한 복령 모두에서 황 성분은 발견되지 않았다. 시설 및 토경 재배 복령을 ICP 분석법으로 11종의 무기질을 분석한 결과 S, Fe, Mg, Zn의 함유량은 1년차, 2년차 모두 토경 재배 복령이 시설 재배 복령보다 많았으며 특히 Fe와 Zn의 함유량은 각각 약 3.1~4.9배, 3.6~3.9배 높았다. 50% 에탄올 복령 추출물의 DPPH와 ABTS radical 소거활성과 FRAP 방법을 실행하여 항산화 효과를 알아보았다. 복령 추출물 10 mg/ml의 농도에서 시설 및 토경 재배 복령의 DPPH IC50 값은 1년산 시설 재배(8.601mg/mL), 1년산 토경 재배(12.85mg/mL), 2년산 시설 재배(1.23mg/mL), 2년산 토경 재배(1.18 mg/mL)로 나타났으며 ABTS IC50값은 1년산 시설 재배(15.85mg/mL), 1년산 토경 재배(14.59 mg/mL), 2년산 시설 재배(3.9 mg/mL), 2년산 토경 재배(14.92 mg/mL)로 계산되었다. 연구에 사용한 복령의 항산화 활성은 농도 의존적인 증가를 보였고 2년산 시설 재배 복령이 가장 높은 항산화 활성을 나타냈다. FE-SEM을 이용하여 복령 입자의 초미세구조를 관찰한 결과 샘플간의 뚜렷한 차이점은 발견되지 않았다.

Since the introduction of product liability law from America in 1960s, product liability has been on the rise as an important problem to the quality management of company and consumer's safety. Together with this, before the legislation of product liability system in Korea, the sense and level of company and consumer about product safety are rapidly changing, In times like the present, ensuring more systematic product safety and consumer safety, and the buildup need of competitive power in accordance with product liability prevention of company grows raising. Therefore, this study presents the most effectively manageable ways of product liability the side of safety management of consumers and companies through the ensuring ways and activity models of product safety and certification system in company.

The present study was carried out to evaluate the antioxidant and anti-inflammatory activities of threeextracts (hot water, 50% ethanol and mixed solvent;water, ethanol, butylene glycol, propylene glycol) of dried chestnut,chestnut shell, chestnut leaves and dried chestnut leaves obtained from Castanea crenata tree. When conducted DPPH assay,radical scavenging activity of ethanol extract of chestnut shell was the highest with IC50 10.8㎍/mL among four extractsfrom these parts (p<0.05). In additional results by the xanthine oxidase assay, antioxidant activity showed that waterextract of chestnut leaves showed the highest xanthine oxidase inhibitory activity in the tested extracts (p<0.05). Futher-more, extracts of chestnut shell and leaves exhibited no cytotoxicity in RAW 264.7 cells (p<0.05). Also, anti-inflammatoryactivity by NO assay showed LPS-induced NO was significantly inhibited following treatment with extracts of chestnut shelland leaves of 3㎎/mL (p<0.05). These data suggest that extract of chestnut shell have antioxidant and anti-inflamantoryactivity including chestnut leaves. Therefore, it is considered that Castanea crenata research range and selection of functionalmaterial can broaden chestnut shell to other fractions such as chestnut and chestnut leaves.