A study was conducted to investigate the effect of supplementing earthworm meal (EWM) on the performance of spent laying hens after induced molting, and fatty acid composition in egg yolks. A total of 90 laying hens at 73 weeks of age were fed the experimental diets containing 0.0% (Control) and 0.2% of EWM for 4 weeks. Eggs were collected and weighted every day and egg production and feed conversion were recorded every week during the experimental period. However fatty acid composition of egg yolk were measured at last week of experimental period. An amount of feed intake increased by supplemental with EMW except for first week experimental period (P＜0.05). When 76 and 77 week of age, egg production and daily egg mass were improved by the supplemented with EWM (P＜0.01), but average egg weight was not different when fed a EWM, therefore feed/egg mass was significantly decreased when fed a EWM. The ratio of egg yolk n-6/n-3 fatty acids contents was significantly improved fed a EMW (P＜0.05). It is concluded that supplementing 0.2% of earthworm meal in the spent laying hens after induced molting was fast in recover of physical strength, therefore improves laying performance.