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        검색결과 2

        1.
        2023.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        We investigated the epidemiological characteristics of the antimicrobial resistant Enterococcus isolates from the four major rivers of Korea in 2012. A total 316 surface water samples were collected from three distinct sites (nearby livestock farms, tributaries, and major rivers) at two different seasons (dry season: n = 76, wet season: n = 240). A total 654 bacterial cells were isolated from samples and their genus distribution were determined. We found that Gram-negative bacteria including various genera were prevalent (n = 522, 79.8%), and Enterococcus was the most common genus of Gram-positive bacteria (n = 119, 18.2%). The isolation rate of Gram-negative bacteria was higher in wet season, whereas that of Enterococcus isolates was higher in dry season. The prevalence of Enterococcus isolates was also higher nearby livestock farms than on tributaries and main rivers. Since Enterococcus isolate is a key indicator for animal fecal contamination, the following experiments focused on this microorganism. As compared to a previous report in 2006, the resistance rates in E. faecium to erythromycin (40.0% to 69.9%) and chloramphenicol (0% to 16.4%) were increased, whereas those to penicillin (56.0% to 4.1%) and teicoplanin (36.0% to 0%) were decreased. We also found that antimicrobial-resistant (AMR) E. faecium isolates from rivers and livestock samples shared similar pulsed-filed gel electrophoresis (PFGE) profiles, validating the transmission of AMR Enterococcus isolates from livestock to river. Taken together, this study provides us with detailed information about bacterial contamination status in four major rivers, and highlights the changes in AMR pattern of Enterococcus isolates, which are expected to have originated from livestock.
        4,000원
        2.
        2012.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Antibiotic resistance in animal isolates of enterococci is a public health concern, because of the risk of transmission of antibiotic-resistant strains or resistance genes to humans through the food chain. This study investigated phenotypic and genotypic resistances profile of tetracycline in 245 Enterococcus isolates from bovine milk. A total of 245 enterococci were isolated from 950 milk samples. The predominant strain was E. faecalis (n = 199, 81.2%) and E. faecium (n = 25, 10.2%). E. avium (n = 7, 2.9%), E. durans (n = 6, 2.5%), E. gallinarum (n = 4, 1.6%), and E. raffinosus (n = 4, 1.6%) were also isolated. Of the 245 enterococcal isolates 76.3% (n = 187) displayed tetracycline resistance (≥ 16 μg/ml). Of the 187 tetracycline-resistant isolates, 83.4% (n = 156), 16.1% (n = 30), and 26.7% (n = 50) possessed the genes tet(M), tet(L), tet(S) respectively. While 3.2% (n = 6) of the tetracycline- resistant isolates possessed all three genes tet(M) + tet(L) + tet(S), 8.6% (n = 16), 16.0% (n = 30), and 2.7% (n = 5) of them possessed two genes tet(M) + tet(L), tet(M) + tet(S), and tet(L) + tet(S) respectively. The tetracycline resistance pattern investigated in this study was attributable mainly to the presence of tet(M).
        4,000원