This study represents a fundamental research for a passenger-ship escape guidance system that is a ship-borne agent, and it is for the support of escape decision-making by providing accurate maritime safety information in real time. We conducted a passenger escaping experiment on a training ship, SAEYUDAL, using the situation-aware escape guidance system, which has been developed for on-board application based on products for buildings. It is shown that the system contributes to the shortening of the escape duration and the dispersion of the escaping persons by generating and guiding the safe escape routes which dangerous areas are considered. From the experiment on ship's heeling condition, it is revealed that the heeling angle is linearly related to the escape duration. Therefore, this study shows that the shortening of the escape duration enables a safe and rapid mustering that is the most important process under emergency and appropriate incident responses to minimize loss of lives.