This study evaluated the effectiveness of odor reduction when spraying inside the Bio-curtain (hereinafter referred to as curtain) according to the exhaust fan operating rate. Spraying is a main factor affecting the ability to odor reduction of curtains. The curtain (total area: 37.9m3) was constructed with two layers of light-shielding screens stretched over a rectangular parallelepiped structure installed around an exhaust fan (630 mm) on the side wall of a pig barn. Air samples for odor analysis were collected from inside the pig barn and outside the curtain. The main odorous compounds such as volatile fatty acids, phenols, indoles, and ammonia were measured. The odor reduction effectiveness was evaluated by total odor activity values (TOAVs) summed to the odor activity values of each odorous compounds. Depending on the exhaust fan operating rate, the reduced rate of TOAVs gradually decreased to the range between 15.67% and 68.80%. Because the contact time between the spraying liquid and the air velocity of the exhaust fan becomes shorter (or there is a reduction in liquid to gas flow ratio) as the exhaust fan operating rate increases. The results of this study can be used as basic data for research into spraying conditions to improve the odor reduction effectiveness of curtains.