The age and growth of the Marbled sole, Pleuronectes yokohamae were investigated using left sagittal otoliths of 832 individuals collected in the mid-southern waters of Korea from January 2006 to April 2007. The seasonal changes in the ratio of an translucent zone to interval between annular rings revealed that the boundaries between translucent and opaque parts of the otolith were annual growth rings formed between May and June once a year. The spawning period was November to March and therefore the duration from fertilization to the complete formation of the annual growth ring became fifteen months. The parameters of von Bertalanffy growth equations estimated using the average standard length at each age are the L∞ of 48.04cm, K of 0.1406/year and t0 of -0.5116 for female and 26.14cm, 0.3056/year and -0.8202 for male, respectively. Finally, it is suggested that the growth of female is faster than that of male from age-2.

The antigen GA733 is a cell-surface highly expressed glycoprotein on most human colorectal carcinomas. GA733 can be characterized as a cancer vaccine. In this study, GA733 was fused to the human immunoglobulin IgG Fc fragment to become recombinant gene GA733-Fc. Based on this, 4 recombinant genes were constructed as follows: GA733-Fc with signal peptide sequence and fusion of ER retention sequence (KDEL) (spGA733-FcK), GA733-Fc with signal sequence (spGA733-Fc), GA733-Fc fused to ER retention sequence (GA733-FcK) without signal peptide and GA733-Fc without signal peptide. Baculovirus-insect cell expression system is widely used for the high level production of recombinant proteins especially for glycoproteins. Constructed 4 recombinant genes were cloned to baculovirus express vectors. DH10Bac E.coli.-mediated transformation was used to generate recombinant bacmid DNA. Recombinant DNA was confirmed by PCR. Insect cell was transfected by bacmid to produce the recombinant baculovirus infects insect cell to produce recombinant protein. Western blot and sandwich ELISA showed the expression of recombinant proteins. Each cell lines (sf9 and HighFive) differed in recombinant proteins production level and protein secretion capability. N-Glycosylation analysis showed the function of signal peptide and ER retention sequence (KDEL). Taken together, baculovirus-insect cell system can be used to express recombinant GA733-Fc glycoproteins.

Immunotherapeutic approaches using agonist antibodies or fusion proteins of immunomodulatory molecules significantly inhibit tumor growth and boost cell-mediated immunity. We isolated mRNA from previously reported 1D4 hybridoma cells and amplified the variable regions of the heavy chain (VH) and light chain (VL) genes using reverse-transcriptase polymerase chain reaction. Using a linker, the amplified sequences for the heavy and light chains were each connected to the sequence for a single polypeptide chain that was designed to be expressed. VL and VH fragments were cloned into the pOptiVEC-TOPO vector containing the human CH2-CH3 fragment. Then, 293T cells were transfected with the 1D4 single-chain Fv-Fc (scFv-Fc) constructs. A549 cells were used for presentation of the 1D4 antigen. Flow cytometry was performed for analysis of the secreted 1D4 scFv-Fc constructs. The DNA sequence of 1D4 scFv-Fc was obtained. The 1D4 scFv-Fc constructs were expressed by the transfected 293T cells and secreted into the culture medium. The immunoreactivity of the secreted scFv-Fc construct was lower than that of the murine 1D4 antibody for A549 cells. A 1D4 scFv-Fc construct for immunotherapy was developed.

본 연구에서는 수원 성균관대학교 내 Frequency Domain Reflectometry (FDR) 토양수분 측정 장비 및 COSMIC-ray 중성자 측정 장비를 통한 토양수분 지점 관측 사이트를 확립하였다. 또한 양질의 토양수분 데이터 확보를 위해 연구지역 내 토질실험, 토질별 FDR 토양수분 데이터 및 COSMIC-ray 중성자 개수의 시계열 분석, 관측한 토양수분 데이터와 위성 기반 토양수분 데이터와의 비교분석을 실시하였다. 2014년도부터 6개 지점에서 표층으로부터 5 cm에서 40 cm까지 총 24개의 FDR 센서를 5~10 cm 깊이별로 설치하여 토양수분 데이터를 측정하였다. 해당 지점들 의 토질 분석결과, Sand에서 Loamy Sand까지의 다양한 토질이 불균질한 층을 이루어 분포되어 있는 것으로 판단되었다. 측정된 토양수분 데이 터는 강우 데이터와 높은 상관성을 보이며, 위성 산출 토양수분 데이터와의 비교에서도 상대적으로 높은 상관관계와 낮은 평균제곱근편차(Root mean square deviation, RMSD)값을 보여주었다. 2014년도 설치 지역 토양수분 데이터의 신뢰도가 확보됨에 따라 2015년도에는 10개의 FDR 토양수분 측정 장비 및 COSMIC-ray 중성자 측정 장비가 추가로 설치되어 성균관대학교의 Soil Moisture site with FDR and COSMIC-ray (SM-FC) 연구지역이 구축되었다. SM-FC에 설치된 COSMIC-ray 중성자 측정 장비의 최초 검증을 위해 2015년 8~11월의 COSMIC-ray 중성자데이터 및 FDR 토양수분 데이터가 활용되었다. 중성자기반 토양수분 값과 전체 지점 FDR 토양수분 평균값을 비교한 결과 매우 높은 상관관계를 볼 수 있었다 (상관계수 0.95). 이러한 연구를 통해 성균관대학교 SM-FC는 향후 한반도 지역 위성 및 모델 토양수분 데이터를 검증하는 대표 연구 지역이 될 것으로 기대된다.

The portfolio selection is one of the most important and vital decisions that a real or legal person, who invests in stock market, should make. The main purpose of this article is the determination of the optimal portfolio with regard to relations among stock returns of companies which are active in Tehran’s stock market. For achieving this goal, weekly statistics of company’s stocks since Farvardin 1389 until Esfand 1390, has been used. For analyzing statistics and information and examination of stocks of companies which has change in returns, factors analysis approach and clustering analysis has been used (FC approach). With using multivariate analysis and with the aim of reducing the unsystematic risk, a financial portfoliois formed. At last but not least, results of choosing the optimal portfolio rather than randomly choosing a portfolio are given.