The FTA card is a quick and easy method to preserve DNA from plant tissues. Its applicability has only been tested in some economically important plants and in research model plants such as rice, potato, tomato, and Arabidopsis. Therefore, DNA preservation with FTA cards in various tracheophytes (196 taxa) was tested in this study based on PCR using DNA barcoding primers. The results indicated that DNAs were successfully preserved with FTA cards in 76.74% of the tested samples and each taxon showed a different preservation rate. Especially, most families in monocots showed high preservation rates (100%) while Asteraceae showed low preservation rate (28.57%) compared to other families. Stored FTA cards were tested again after five years under the same experimental conditions as the previous test. The results showed that DNA was preserved in 54.26% of the samples, indicating that 29.9% of DNA-preserved samples are degraded after five years. However, Araceae, Amaryllidaceae, Asparagaceae, Liliaceae, Melanthiaceae, Brassicaceae, Araliaceae, and Asteraceae samples, of which DNA preservation was confirmed five years earlier showed a 100% preservation rate. This study suggests that the FTA card is a useful tool for DNA preservation in various plant taxa, of which preservation is confirmed in this study (or closely related taxa) and is applicable in the field of population genetics, phylogeny, identifications, inspections, etc.