Purpose: The present study examined whether golf mindfulness routine (GMR) enhances golf performance (objective and subjective), taking into consideration the effects of dispositional mindfulness. Methods: Five male high-level golfers participated in the study. Basic procedures of GMR were consisted with three phases: a) awareness of environmental conditions, b) focusing on body-mind conditions c) monitoring and accepting emotions in present. A single-subject design (changing conditions design) was used to determine the effects of GMR over three sessions (pre, post, follow up). Results: The results were provided for the effectiveness of GMR on golf performance in all golfers. GMR also fostered dispositional mindfulness of golfers over intervention sessions. Conclusion: The theoretical significance of the results is assessed and the application of GMR in golf performance enhancement is discussed.