Avian Influenza (AI) is an avian disease that break out by AI virus (AIV). As Hemagglutinin (HA) that is a main antigen surface protein of influenza virus that causes these influenza infection changes continuously, HA escapes bond of guided antibody by host"s immunity system. Specification region (HA91-261) of HA molecule that reconize receptor (sialic acid) of host cell is well-preserved without changing relatively, but is gotten buried on inside of trimer structure in natural conditions, is reported that development of effective vaccine or cure for HA protein is difficult because is not exposed to immunity system. Aptamer is relatively small strand DNA or RNA, and has high affinity to specific proteins. In this study, new aptamer DNAs were screened which can specifically bind to the receptor binding region of HA H9 protein.