The Hawthorn spider mite Amphitetranychus viennensis is an important pest of various fruit trees as well as a variety of ornamental plants. Its presence has been reported in China, Japan, Korea, and middle east and European countries including Grorgia, Russia, Turkey, Ukraine. Thus, it is a quarantine species in American countries and Australia. Intra-specific variation of A. viennensis was investigated using 555 bp of ITS2 ribosomal DNA and 453 bp of the cytochrome oxidase I (COI) gene. Its variation of ITS2 and COI among China, Japan and Korea were 1.7-2.2% and 0.2-0.7%, respectively. In addition, we collected samples from 14 location in Korea. Intra-specific diversity of ITS2 and COI within Korean populations were ranged into 0.5-0.9% and 0.2-0.5%, respectively. Thus, A. viennensis is not much genetically diverse in different populations.

 ,  , The hawthorn spider mite, Tetranychus viennensis, is a pest of apples and a quarantine pest from some countries that import apples from Korea. A controlled atmosphere and temperature treatment system (CATTS) was developed as an alternative disinfestation method to methyl bromide fumigation treatment, and has been applied to control various insects and other arthropod pests on fruits. We applied CATTS to disinfect T. viennensis under conditions that were previously developed to control the peach fruit moth, Carposina sasakii. First, T. viennensis was sampled from Japanese apricot, Prunus mume, and identified by its morphological characters. In addition, both cytochrome oxidase I (COI) and internal transcribed spacer (ITS) sequences supported the morphological identification. Second, the heat-tolerant developmental stage was determined in T. viennensis. When a 46℃ heat treatment was applied to egg, nymph, and adult stages of T. viennensis, adults were the most tolerant stage. Third, when heat temperature was used along with 1% O<, SUB>, 2<, /SUB>, and 15% CO<, SUB>, 2<, /SUB>, , the mites showed a significant increase in susceptibility to the heat treatment. Finally, CATTS at 46℃ with 15% CO<, SUB>, 2<, /SUB>, and 1% O<, SUB>, 2<, /SUB>, for 30 min resulted in 100% mortality of all T. viennensis development stages. These results indicated that CATTS isapplicable to disinfest T. viennensis in post-harvest apples.