The objective of this study was to detect and identify the bee mites that were related to the colony collapse disorder of the western species of honey bee. From July 6, 2023 to August 2, 2023, total 367 bee larvaes and 243 adults bee were observed by 100-fold and 200-fold magnification microscopy. As a result of direct visual and microscopic examination on honeybee bodies, larvae and pupae, a total of 2,688 bee mites were found. Of their bee mites, it were 9 of Tropilaelaps clareae, 130 of Varroa jacobsoni, and 2,549 of Varroa destructor, respectively. Tropilaelaps clareae showed 7 females, 1 male, and 1 larva, Varroa jacobsoni had 94 females, 5 males, and 31 larvae, and Varroa destructor had 2,162 females, 346 males, and 41 larvae, respectively. In order to reduce the damage of the apiary caused by bee mites, it is mainly dependent on pharmaceutical control, continuously.

Varroa destructor is a devastating ectoparasitic mite which attacks Honeybee, Apis mellifera. V. destructor feeds on honeybee hemolymph, and often harbors small RNA viruses such as the deformed wing virus to transmit these viruses in the infested bee hive. To survey the genes of V. destructor, total RNA was subjected to high-throughput transcriptome sequencing to construct in silico cDNA library by using the Illumina HiSeq 2000 platform. Total of 2×107,748,792 paired-end short reads were obtained and quality filtered reads were subjected to Trinity de novo assembler followed by TransDecoder, and CD-HIT program to make a V. destructor reference cDNA library containing 28,023 of clustered contigs with protein coding capacity. These cDNA sequences will help us to understand the molecular biology of V. destructor.