Background : The roots of Codonopsis lanceolata have been used as a tonic crude drug and an edible plant in Korea. The plant mainly contains triterpenoid saponins, including codonolaside, codonolasideⅠ-Ⅴ, lancemaside A-G. Their saponins have shown anti-inflammatory effects such as bronchitis and cough, insomnia and hypomnesia. C. lanceolata is well known to affect various pharmacological effects for human health, and its consumption is increasing. Recently, plant and plant-derived products were treated a part of the healthcare system by applying the bioactive phytochemicals. Antioxidant and immune activity substances in food play an important role as a health-protecting factor. This study was designed to investigate the in vitro immune cell growth and xanthine oxidase Inhibitory activity of different storage period and storage temperature of C. lanceolata. Methods and Results : The plant materials were used the roots of C. lanceolata cultivated in Jeju area, Korea. Immune enhancing effect was conducted using T cell and B cell of human immune cells. Each cell incubated for 8 days with the sample extracts compared to the control group, and the immune activation was measured according to the growth of immune cells. The xanthine oxidase Inhibitory activity was measured by modifying the method of Noro(1983). In different storage period and storage temperature conditions, the immune cell growth of C. lanceolata extract promoted a concentration-dependent manner in both human T cell and B cell, and did not show a significant difference. The xanthine oxidase Inhibitory activity of C. lanceolata extract tended to decrease more, depending on the longer the storage period or the higher the storage temperature. Conclusion : These results of this study suggested that the root of C. lanceolata may assist in the potential biological activities, and can be used as a source of human health products.