Lord's Holly tree(Ilex rotunda) has shown different rooting rates depending on the time and conditions of cutting. The best result, 100 percent of rooting, could be accomplished in early November when cuttings were done on the mixture-soil containing the same rates by volume of vermiculite, peatmoss and perlite with cuts processed with IBA 1,000 mg/l + NAA 1.000 mg/l under the circumstances that 70% of shade, plastic mulching, and mist were conditioned. Even though cuttings processed with plant growth regulator, IBA 1,000 mg/l, showed a little bit lower rate of rooting than those processed with IBA 1,000 mg/l + NAA 1,000 mg/l, it should be considered that the former was better than the latter regarding the number of roots and root length. However it should be further studied about how to utilize re-juvenile cutting of adult trees to propagate in a large quantity female Ilex rotunda which propagates in dioecism.