This study analyzed symptoms of MSDs(Musculokeletal Disorders) associated with characteristics of smartphone usage based on questionnaire survey. This study also tried to identify their relationship based on the results from questionnaire survey. A total of 943 subjects participated for the survey. The results in terms of symptoms associated with smartphone usage were as follows; 1) 49.8% of respondents experienced symptoms associated with MSDs, 2) 35.9%, 34.0%, 22.1%, 21.7% and 20.9% of respondents had symptoms at shoulder, neck, elbow, wrist, and fingers respectively, 3) The effect of ‘daily time of data use’, ‘monthly costs’ for MSDs of respondents was significant. Specifically, this study has some significance since it tries to identify the relationship between MSDs symptoms and characteristics of smartphone usage. The results of the study can be good basis for better design and user guidelines of smartphone to prevent MSDs associated with smartphone usage.