Koreoleptoxis nodifila (Martens, 1886) is an endangered species only living in the central and north streams of South Korea. However, there is a lack of information on physical habitat characteristics of K. nodifila. We aimed to determine preference ranges for water depth, current velocity, streambed substrate of K. nodifila. The weibull model was used to estimate the habitat suitability based on distribution of individual abundance by physical factors. Optimal depth preferences ranged from 0.53~17.17 cm, current preferences ranged from 48.40~81.03 cm s-1 and substrate (Φm) preferences ranged from - 4.36~ - 2.26. Median values of central tendency were determined as follows: water depth 16.73 cm, current velocity 65.23 cm s-1, substrate - 3.51. Mean values of central tendency were determined as follows: water depth 21.32 cm, current velocity 65.65 cm s-1, substrate - 3.63. Mode values of central tendency were determined as follows: water depth 5.17 cm, current velocity 64.77 cm s-1, substrate - 3.24. Based on the habitat suitability analysis, the microhabitat types of K. nodifila were determined as riffle and coarse-grained streambed.