This study aims to empirically investigate effects of motion repetition and external load according to joint motion on discomfort through an experiment. Eighteen college students (female: 9; male: 9) participated in an experiment measuring perceived discomfort using the Borg CR10. Joint(wrist, elbow, shoulder and trunk) motion, its repetition and external load were adopted as independent variables. The results showed that all three independent variables were statistically significant on discomfort. Participants’ sex also significantly affected discomfort obtained in the experiment. While the interactions of joint motion and repetition, and joint motion and external load were not significant at α = 0.05, that of motion repetition and external load was significant. Based on the experimental results, four regression equations by the joints involved were presented, which could be used as a tool for evaluating postural loads by the joints. It may be postulated that based on the results of this study, scoring systems of RULA and REBA, and those of OWAS and REBA underestimates effects of motion repetition and external load, respectively. It is expected that the results of this study will be used as a basic data for developing an observational method properly reflecting the effects of motion repetition and external load.