A new near loss-less compression method for 2D game sprite animation image is presented to reduce the amount of the game contents resources. The sprite animation data in 2D mobile game occupies a large part of the resource, and in particular the sprite animation data by using a lossless compression technique can cause a relatively very significant increase of data amount as the higher complexity of the animation. The new near-lossless method, which is an adaptive compression technique by considering the alpha value for transparency and the variance of color values of block, is proposed in order to obtain greater compression efficiency without los of quality. By using experiments, we show that the proposed method compared to the PNG compression technique is good for compression efficiency, and in particular the effect is very high in complex and dynamic 2D sprite animation.