느타리버섯 재배시 기존의 고압살균처리는 영세한 버섯재배 농민의 시설비나 운영면에서 비용이 많이 들어 야외 발효나 저온살균에 의한 저비용 고효율의 최적배지 선발을 위한 살균방법별 시험결과를 요약하면 가. 배지 재료에서는 톱밥＋면실＋왕겨 (1:1:1)배지와 톱밥＋면실＋비트 (5:3:2)처리가 비슷한 경향이었고 나. 살균방법 처리에서는 고압살균(121℃, 90분) 방법보다 저온살균(50­60℃, 7일)처리가 균사배양 및 생육특성이 양호하였으며 다. 기존의 고압살균 톱밥＋면실＋비트(5:3:2)처리구 174g/1.5kg 대비 저온살균 톱밥＋면실＋왕겨 (1:1:1) 205g/1.5kg으로 18%증수되고 톱밥＋면실＋비트(5:3:2) 처리구에서는 207g/1.5kg으로 19% 증수되는 경향이었으나 톱밥＋면실에 왕겨를 첨가하나 비트펄프를 첨가하나 유의성이 없었다. 라. 봉지 재배시 저온살균이 고압살균대비 경영비 12% 절감과 농가소득 향상에 기여 할 것으로 기대된다

This study was carried out to find a useful mushroom at Chungnam Agricultural Research And Extention Service. Twenty materials used were collected from domestic and exotic area. These races were compared bontanical characteristics to leading varieties by PCR-RAPD methods. Mycelial growth temperature of Chongpung and Myongwol were at 20 to 25 ℃ and 25 to 30 ℃ at PDA medium, respectively mycelial growth of these varieties were similiar at pH 6.5 to 7.5. In case of mushroom cultivation temperature ranges, Chongpung was at 5 to 26℃ and Myongwol was at 7 to 28℃, but the optimum temperature range of these were appeared at 15 to 19℃. Culture temperature of these was 23℃ and period of mycelial culture was needed 23 to 24 days under 850cc/pp, while was needed 11 to 12 days at waste cotton medium. Cap color of these at first inducing mushroom was all dark blue, but at late growing stages Chongpung was shown as grey, and Myongwol was shown as dark grey. Yield of Chongpung was appeared as 46kg/3.3m2 and that of Myongwol was 41kg /3.3m2, while Chunchu No2 as check was 40kg/3.3m2. Results from PDA medium and PCR-RAPD analysis two of these were different from others.