This study was conducted to distinguish adult morphology of the giant water bug (Lethocerus deyrollei) between over-wintered (OLD) and new-born (NEW) populations using materials collected from Gyodong island in Gyeonggi-do, Korea, as well as individuals reared in the laboratory (LAB population) as a control. As a result, no fundamental differences were found in external body structure between OLD, NEW, and LAB populations. However, marginal area of basal procoxa in ventral prothorax and jointed part of forefemur and foretibia were greatly worn in the OLD population. Mid-claws were shorter than 0.5 times the length of the 2nd mid-tarsus, the membranous margin of hemelytra was greatly worn, and hairs of the 5th abdominal tergum were greatly reduced in the OLD population.