This study was conducted to investigate visual threshold of nigro (Cichlasoma nigrofasciatum) on white LED light. Thevisual threshold was obtained by analyzing electrocardiogram (ECG) of the nigro. 5 individuals (body weight: 15.62~45.49 g; TL: 8.9~12.4 cm) were trained for lights by an electric stimulus. And then the heart rate (beats/10s) before and after switching on the light were compared. Light intensity range was from 0.00 to 226.4 lux. Average heart rate was 10.36 beats/10s in the normal condition. When the fish perceived the light, the heart rate was decreased. Visual threshold of the fish was 2.59 lux.