본 연구의 목적은 질산, 황산, 암모니아수, 과산화수소에 대한 생태독성평가를 통해서 사고대비물질들에 대한 기초 독성 데이터베이스를 구축하여, 향후 화학사고 발생시 환경 피해에 관한 의사결정에 과학적 근거를 제공하는 데 있다. 이를 위해 본 연구에서는 사고대비물질 중 토양의 물리·화학적 성질을 변화시킬 수 있는 질산, 황산, 암모니아수, 과산화수소를 대상으로 국내 토착 절지동물인 김어리 톡토기 (Paronychiurus kimi)를 이용한 생태독성평가를 수행하였다. 7일간의 급성독성평가와 28일간의 만성독성평가를 수행하였으며, 시험물질 농도에 따른 토양의 pH 변화를 관찰하였다. 토양의 pH는 질산, 황산, 과산화수소, 암모니아수의 농도가 10,000 mg kg-1 soil dry wt.일 때, 각각 2.86, 2.72, 7.18, 9.69이었다. 질산, 황산, 과산화수소, 암모니아수에 대한 만성독성평가 결과, LC50 값은 각각 2,703, 5,414, 3,158, 859 mg kg-1 soil dry wt.이었으며, P. kimi의 산란 수에 대한 EC50 값은 각각 587, 2,148, 1,300, 216 mg kg-1 soil dry wt.이었다. 비록 본 연구에서는 사고대비물질들의 유입에 따른 토양 pH의 변화만이 조사되었지만, 본 연구의 결과는 P. kimi가 사고대비물질에 의해 변화된 토양의 pH뿐만 아니라 사고대비물질의 유입에 의해 감소된 유기물 함량과 생성된 반응 산물에 의해서도 사망률과 산란수에 영향을 받을 수 있음을 의미한다. 대부분의 사고대비물질들이 토양의 특성을 변화시킬 수 있다는 점을 감안할 때, 토양의 특성 변화와 이에 따른 생물 영향을 고려한 화학사고 후 평가 및 복원 방법이 필요하다.

Acute and chronic toxicities of methyl ethyl ketone and methanol were investigated on Paronychiurus kimi (Collembola), for evaluating the potential effects of accidental exposures of these chemical substances on the terrestrial environments. This study was undertaken to establish a toxicity database for these chemical substances, which was required for the preparation of the response compensation and liability act for agricultural production and environmental damage. The 7-d acute toxicity and 28-d chronic toxicity were conducted using the OECD artificial soil spiked with varying, serially diluted concentrations of methyl ethyl ketone and methanol. Mortality was recorded after 7-d and 28-d of exposures, and the number of juveniles were determined after 28-d of exposure in the chronic toxicity test. In both assessments, methanol was more toxic than methyl ethyl ketone in terms of mortality (LC50) and reproduction (EC50). The 7-d LC50 of methyl ethyl ketone and methanol were 762 and 2378 mg kg-1 soil dry wt., respectively, and the 28-d LC50s were 6063 and 1857 mg kg-1 soil dry wt., respectively. The 28-d EC50 of methyl ethyl ketone and methanol were 265 and 602 mg kg-1 soil dry wt., respectively. Comparison of results obtained in this study with literature data revealed that P. kimi was more sensitive to methanol than other soil invertebrates. However, given the high volatility of the chemicals tested in this study, further studies are necessary to improve the current test guideline, or to develop new test guidelines for an accurate assessment of chemicals that require toxicity databases for chemical accidents.

A new species of the genus Onychiurus is described from Korea(Jeoksang Mountain in Muju). The genus Onychiurus was elected by Gervais in 1841 with type species Podura ambulans Linneus, 1758. 49 specis of Onychiurus have been reported worldwide, of which only one species, O. polychaetosus, have been recorded from Korea. This genus is characterized by postantennal organ with compound vesicles, antennal Ⅲ sense organ with 2 sensory clubs, furca reduced to a small area of fine ganulation with 2+2 posterior setulae arranged in a row, seta d0 on a head and anal spines present. Onychiurus sp.1 is characterized by the peculiar distribution of dorsal (32/0,2-3,2-3/33333) and ventral (1/000/0101) pseudocelli that allow to distinguish it from the other congeneric species. Antennal segment Ⅲ with four guard setae, two sensory rods, two bent and granulated sensory clubs and five papillae irregular in shape. Postantennal organ consists of 10-12 finely granulated vesicles and tibiotarsi with distal whorl composed of nine setae.

Arsenic is a naturally occurring element in the earth's crust and is found throughout the environment, but compared with other toxic elements, little toxicological work has been conducted on arsenic. In this study, we tested the effect of two different arsenic compounds (arsenite (NaAsO2) and arsenate (Na2HAsO4)) on the reproduction of soil inhabiting collembolan species (Paronychiurus kimi). The toxicity tests for each arsenic compound were conducted in accordance with the ISO 11267. The estimated EC50s for reproduction of each arsenic compound were 20.43 (16.97-24.60) and 32.86 (25.64-42.13) mg/kg, respectively. There were statistically differences in the toxicity of two different arsenic compounds based on overlapping 95 % confidence intervals. The relative toxicity ratio of arsenite to arsenate (approx. 0.62) as well as the concentration-dependent decrease in reproduction of P. kimi was steeper at arsenite than that of arsenate. These results show that arsenite was more toxic to P. kimi than arsenate.