Purpose: The purpose of this study was to develop a stress test for students in physical education high schools and to verify the validity of the developed test. Methods: To develop the test, this study referred to the stress factors of students in physical education high schools, analyzed by Lee, Dong & Lee(2009) and designed a preliminary test with 42 questions of 7 factors. On the basis of the data of 544 students in three physical education high schools, this study conducted exploratory and confirmatory factor analyses to examine the structure and goodness-of-fit of the data, and performed reliability analysis. To verify the convergent validity of the confirmed test, it used the data of 82 students in one physical education high school. Lastly, based on the data of 626 students, it conducted factor invariance test and latent means analysis to investigate the difference between background variables. Results: It was found that the stress test for students in physical education high schools had the structure of four factors: athletic stress, conflict with teammates, coach stress, and life stress. According to the comparison between the test and other tests in terms of convergent validity, the developed test explained construct well. Factor invariance of each background variable was satisfied. According to the analysis of the difference between groups, women had more stress than men in terms of athletic stress and coach stress. The 1st year students had more conflict with teammates than the 2nd year students, and the 3rd year students had high athletic stress, coach stress and life stress.