The purpose of this study was to determine the optimal digestible energy levels on pregnancy mares. Physical changes and fecal microorganism in pregnant horse and changes in birth characteristics of neonatal foals were investigated. The experiment was conducted by 18 mares (Jeju corssed bred, older than 36 months) into three treatment groups. The experimental diet consisted of 80%, 100%, 120% digestible energy levels based on NRC. The average daily intake was lower in the 120% (8.75±1.01) than the 100% (9.34±0.92), 80% (9.14±0.88) and there was significant difference (p<0.05). The feed efficiency was lower in the 120% than 80%, 100% (p<0.05). Total cholesterol, HDL-cholesterol, LDL-cholesterol and triglyceride was higher in 120% than others (p<0.05). However there were no health problem and there was no difference between the treatment groups in the birth characteristics of neonatal foals. At the phylum level, Fibrobactres was difference by digestible energy levels, 80% (8.53%) was higher than 100%, 120%. At the genus level, Bacteroides and Kineothrix increased in fecal proportions with increasing digestible energy levels (p<0.05). Fibrobacter showed higher composition at 80% than 100% and 120% (p<0.05).