In the integrated ancient East Asian sphere, literature is an explicit expression of unity. However, due to differences in perspectives, there are huge contrasts and disharmony in contemporary East Asia surrounding historical issues. Using artificial intelligence, specifically the retrieval-enhanced generative model, to build an intelligent research platform for Yanxinglu, and completing research auxiliary work that includes named entity recognition, relationship extraction, and knowledge graph construction, the study of East Asian history can be enhanced. This paper focuses on the construction of the LLM-RAG model and rules in the construction of the Yanxinglu knowledge base, and discusses the time process and precautions for the refined processing of the Yanxinglu text data.

Fish larvae are immediately exposed to microbes from hatching to maturation of their lymphoid organs, therefore effective innate mechanisms is very important for survival. However, the knowledge of the development of immune system in fish is limited and in demand now. In vertebrates, recombination-activating gene 1 (RAG-1) and immunoglobulin M (IgM) have been considered as very useful markers of the physiological maturity of the immune system. In this study, the expression of the both genes was assessed throughout the early developmental stages of olive flounder larvae (5-55 dph) and used as markers to follow the development of immune system. RAG-1 and IgM mRNA expression was detectable at 5 dph and remained so until 55 dph. These patterns of expression may suggest that the olive flounder start to develop its function around 5 dph. Tissue distribution was found that both genes mRNAs are only expressed in the immune-related organ such as spleen, kidney and gill. The early detection of IgM mRNA led to the investigation of its presence in oocytes. Both RAG-1 and IgM mRNA transcripts were detected in unfertilized oocytes, suggesting that they are maternally transferred. The biological significance of such a phenomenon remains to be investigated.