Rock bream iridovirus (RBIV) is a megalocytivirus widely infected in various fish species in Korea, causing symptoms of acute inflammation and enlargement of spleen. In our previous study, RBIV induced the initial upregulation but later down-regulation of proinflammatory cytokines and IFN1 gene expression. Signal transducers and activators of transcriptions (STAT) are transcription factors involved in the regulation of immune genes including IFNs. This study was conducted to analyse the expression of STAT2. The expressional study of STAT2 gene was performed in head kidney and spleen upon RBIV infection and immune stimulants like LPS or poly I:C in vitro. Consequently, STAT2 gene expression pattern was different in head kidney and spleen as it was significantly up-regulated by LPS from 4 h to 8 h but down-regulated at 24 h while up-regulated by poly I:C at 8 h in head kidney while, in spleen, STAT2 gene expression was down regulated by LPS but significantly up-regulated by poly I:C. Upon RBIV stimulation, STAT2 gene expression was significantly down-regulated by high dose RBIV at 4 h but up-regulated at 8 h and 24 h in head kidney. In spleen cells, it was up-regulated by medium dose RBIV at 4 h and by high dose RBIV at 4 h and 8 h but down regulated later then. In vivo, STAT2 gene expression was not significantly affected by RBIV infection while significant up-regulated by vaccination at day 7 post-vaccination, indicating STAT2 gene can be involved in adaptive immune response in rock bream.

우리나라 전통 초가삼간의 1/4 축소모델을 제작하여 암반지반 조건에 대한 내진성능실험을 수행하였다. Nahanni 지진파를 사용하여 최대 가속도 0.1g~0.6g 범위에서 거동을 분석하였다. 탄성한계에서 초가삼간의 고유진동수는 장방향의 경우 약 1.66Hz, 단방향의 경우 215Hz이다. 탄성한계에서의 감쇠비는 약 7%이다. 수평방향의 가속도응답은 입력에 비해 감소하며 입력가속도 수준이 증가할수록 감소율이 증가한다. 이는 사개맞춤으로 만들어진 목조 프레임의 비선형.비탄성 특성 때문이다. 전통 초가삼간 가옥은 고진동수가 지배적인 암반지반에서 매우 큰 내진성능을 나타내었다.