In this study, we used flow a cytometric assay to evaluate plasma membrane integrity and mitochondrial activity in post-thawed sperm that was supplemented with ginsenoside-Rg1. Varying concentrations of ginsenoside-Rg1 (0, 25, 50 and 100 μM/ml) were used in the extender during cryopreservation to protect the DNA of thawed sperm, thereby increasing the viability and motility rate as evaluated using a computer-assisted sperm analysis (CASA) method. The results derived from CASA were used to compare the fresh, control, and ginsenoside-Rg1 groups. Sperm motility and the number of progressively motile sperm were significantly (p<0.05) higher in the 50 μM/ml ginsenoside-Rg1 group (61.0±4.65%) than in the control (46.6±7.02%), 25 μM/ml (46.2±4.76%), and 100 μM/ml ginsenoside-Rg1 (52.0± 1.90%) groups. However, the velocity distribution of post-thawed sperm did not differ significantly. Membrane integrity and MMP staining as revealed using flow cytometry were significantly (p<0.05) higher (91.6±0.82%) in the 50 μM/ml ginsenoside-Rg1 group than in the other groups. Here, we report that ginsenoside-Rg1 affects the motility and viability of boar spermatozoa. Moreover, ginsenoside- Rg1 can be used as a protective additive for the suppression of intracellular mitochondrial oxidative stress caused by cryopreservation.

전세계적으로 널리 복용되고 있음에도 불구하고 인삼이 중추신경계에 미치는 효과에 대한 세포수준에서의 증거는 별로 없다. 따라서 본 연구자들은 지금까지 보고된 30여종 이상의 ginsenosides 중에서 인삼 효과의 주된 활성성분으로 알려져 있는 Rb1과 Rg1을 이용해 해마신경전구세포의 분화와 증식에 미치는 효과를 연구하였다. 증식실험결과 Rb1과 Rg1을 3일 동안 해마신경전구세포에 처리하면 대조군에 비해 BrdU(+)세포수가 상당히 감소한 반면에 분