A new strawberry cultivar ‘Misohyang’ was originated from the cross between ‘Darselect’ and ‘Wongyo 3111’ in 2009. Among hybrids, ‘Misohyang’, a superior individual possessing vigorous growth, capacity to early differentiate flower buds, and excellent physiological characteristics, was selected. Several tests conducted from 2012 to 2014 revealed that this cultivar was suitable for forcing culture due to its faster flowering and harvest time than ‘Seolhyang’. Fruits of ‘Misohyang’ were conical in shape with a red color, which turned dark red color when fruits were fully ripened. Although sugar contents in ‘Misohyang’ fruits were lower than those in ‘Seolhyang’, their aromatic properties were excellent in terms of synergy effect on perception of sweet flavor. In addition, ‘Misohyang’ fruits could maintain high hardness despite a warm temperature during spring. These physiological characteristics of fruits contribute to its high yields in spring, with an average fruit weight of 20 g. Regarding disease resistance, ‘Misohyang’ showed sensitivity to powdery mildew during nursery period and fruit ripening stage. Since the cultivar ‘Misohyang’ has an excellent flavor with attractive red color, sustainable hardness, and high yields in spring, it is expected to be popularly consumed not only for its fresh fruits, but also for its processed fruits.

우리나라 딸기 재배는 시설 재배로 이루어지고 있으며, 대부분 농가의 급액 관리는 재배자의 경험을 토대로 타이머 제어 방식으로 이루어지고 있다. 타이머 급액 방법은 재배 환경, 작물의 생육 단계, 배지 수분 함량 등을 고려하기 어려워 작물을 최적 수준으로 관리하지 못하고, 급액 관리의 정확성이 결여되는 문제점이 있다. 적산 일사량과 배지 수분함량을 이용한 급액 방법은 작물의 생육 상태에 따라 정밀하게 양액을 공급하는 친환경적인 방법이다. 본 연구는 코이어배지를 이용 한 딸기 수경재배에서 적산일사량과 배지 수분함량을 이용한 복합 급액 제어와 타이머 제어 급액 방법을 비교하고 복합 급액 제어 시 최적의 적산 일사량 기준을 설정하고자 수행하였다. 적산일사량 급액 방법은 외부 일사량을 기준으로 100, 150, 250J∙cm -2에 도달하면 자동으로 급액하며 배지 수분함 량이 60% 미만이면 강제 급액하고, 1회 급액량은 50mL로 공급하였다. 타이머 제어는 대조구로 설정하였다. 급액을 개시 하는 적산 일사량 기준이 작을수록 일일급액량이 많았으며 100J∙cm -2 기준 급액 시 급액량은 250J∙cm -2 처리구 대비 46% 많았다. 지상부 생체중과 건물중 모두 복합 급액 제어 방법이 타이머 제어보다 높았으며, 100, 150J∙cm -2 처리구에서 지상부 생체중이 높았고 100J∙cm -2 처리구에서 건물중이 유의하게 높은 값을 나타냈다. 수량 또한 타이머 제어 방법보다 복합 제어 방법에서 유의하게 높았으며 적산 일사량 기준이 작을수록 초기 수량이 증가하였다. 평균 과중은 타이머 제어 급액 시 가장 낮았다. 본 연구 결과 딸기의 정밀 급액 관리를 위하여 적산 일사량과 배지 수분 함량 센서를 이용한 복합 제어 활용 가능성을 확인하였다.

Fusarim oxysporum에 의한 덩굴쪼김병은 수박 재배에 큰 피해를 일으키는 중요한 병이다. 이 병을 방제하기 위해 덩굴 쪼김병 저항성 박 대목을 이용하는 것이 가장 좋은 방법이다. 본 연구는 박 덩굴쪼김병 저항성 자원을 선발할 수 있는 방법을 확립하기 위해 수행하였다. 효과적인 접종법을 개발하기 위해 접종 후 온도(15, 20, 25, 30°C), 접종 농도(1 X 105, 5 X 105, 1 X 106 and 5 X 106 conidia･mL-1), 접종 시기(파종 7, 10, 13, 16일 후)를 조사하였다. 박 덩굴쪼김병을 접종하였을 때 접종 후 배양 온도나 파종 후 접종 시기의 영향이 적었다. 그러나, 덩굴쪼김병에 저항성인 박의 경우 접종농도가 높을수록 발병이 심하게 나타났다. 따라서, 박 덩굴쪼김병에 대한 효과적인 접종법으로 파종 10일 된 유묘 뿌리를 1 X 105 - 1 X 106 conidia･mL-1 농도로 30분간 접종한 후 감염되지 않은 토양에 옮겨 심고 25°C에서 3주 동안 재배하는 것을 제안한다.

전극 구성에 따른 고효율 전해정련공정의 일반적인 모델을 구축하기 위하여, 상미분방정식 인터페이스를 갖는 COMSOL Multiphysics V5.3 전착 모듈을 사용하여 수치해석을 실시하였다. 구축된 모델은 한국원자력연구원에서 제작한 실험실 규 모 (1kg 우라늄/day 규모) 다중배열전극 전해정련장치를 사용해 전극 간 거리, 전극 배열을 변수로 하여 실시한 실험의 전 류밀도-전위 곡선과 비교하였다. 공정온도는 500℃이다. 용융염은 3wt% UCl3가 포함된 LiCl-KCl 공융염을 사용하였다. 검 증된 모델을 이용하여 전류밀도-셀전위 곡선을 계산한 결과 전극 간 거리가 가까울수록, 전극 배열은 양극/음극 면적비가 증 가할수록 셀 전위가 낮아져 전해정련장치의 우라늄 처리효율을 향상시킬 수 있다는 것을 확인하였다. 이러한 접근은 고출력 사용후핵연료 전해정련기의 안전설계를 위한 데이터베이스 구축에 유용할 것이다.

This study was aimed to evaluate the dose-response the effects of nano-encapsulated conjugated linoleic acids(CLAs) on in vitro ruminal fermentation profiles. A fistulated Holstein cow was used as a donor of rumen fluid. Nano-encapsulated CLAs(LF, 5% of nano-encapsulated CLA-FFA; HF, 10% of nano-encapsulated CLA-FFA; LT, 5% of nano-encapsulated CLA-TG; HT, 10% of nano-encapsulated CLA-TG) were added to the in vitro ruminal fermentation experiment. In the in vitro ruminal incubation test, the total gas production on incubation with nano-encapsulated CLAs was increased significantly according to the incubation time, compared with the control(p<0.05). The tVFA concentrations on addition of LF and HT were significantly higher than that of the control(p<0.05). Thus, nano-encapsulated CLAs might improve the ruminal fermentation characteristics without adverse effects on the incubation process. In addition, the population of Butyrivibrio fibrisolvens which is closely related to ruminal biohydrogenation was increased by adding HT, while decreased by adding LF at 12 h incubation. These results showed that nano-encapsulated CLA-FFA could be applied to enhance CLA levels in ruminants by maintaining the stability of CLA without causing adverse effects on ruminal fermentation profiles considering the optimal dosage.

In this paper, we take into account topology optimization problems considering spatial randomness in the material property of elastic modulus. Based on 88 lines MATLAB Code, Monte Carlo analysis has been performed for MBB(messerschmidt-bo lkow-blohm) model using 5,000 random sample fields which are generated by using the spectral representation scheme. The random elastic modulus is assumed to be Gaussian in the spatial domain of the structure. The variability of the volume fraction of the material, which affects the optimum topology of the given problem, is given in terms of correlation distance of the random material. When the correlation distance is small, the randomness in the topology is high and vice versa. As the correlation distance increases, the variability of the volume fraction of the material decreases, which comply with the feature of the linear static analysis. As a consequence, it is suggested that the randomness in the material property is need to be considered in the topology optimization.

딸기의 내저온성 품종육성을 위한 우수 교배친 선발을 위해‘다홍’,‘감홍’,‘매향’,‘설향’을 자방친 으로 이용하고‘다홍’,‘감홍’,‘매향’,‘설향’,‘수경’,‘선홍’,‘원교3111호’등을 화분친으로 하여 24 조합을 작성하고 조합능력 검정을 실시하였다. 그 결과, 자방친을‘다홍’으로 하였을 경우에는 전체조 합에서 과중이 작아지는 경향이 있었고,‘감홍’은 생존율이 낮아 두 품종은 자방친으로 적합하지 않은 것으로 나타났다. 화분친에 있어서도‘선홍’을 화분친으로 이용한 경우 경도가 낮아지는 경향이 있었 고,‘수경’은 기형과가 다소 발생하는 경향이 있어 화분친으로 적합하지 않았다. 그러나‘매향’과‘설 향’을 자방친으로 이용할 경우 발아율과 생존율이 상대적으로 높았고, 과실품질이 우수하고 수량성도 높은 것으로 나타났다. 따라서‘매향’과‘설향’이 자방친으로 우수하였고, 화분친으로는‘매향’,‘설 향’,‘원교3111호’등이 우수하였다. 특히,‘매향’ב설향’,‘설향’ב매향’,‘설향’ב원교3111호’조합 은 당도, 과실크기, 경도, 수량성 측면에서 우수성이 인정되기 때문에 내저온성 교배조합으로 적합하다 고 판단된다.

본 논문에서는 반강접을 고려한 프레임 구조에서 강재 탄성계수의 불확실성이 프레임 구조의 비선형거동에 미치는 영향을 분석하였다. 강재 탄성계수의 불확실성의 확률분포는 정규분포로 모델링 하였으며, 이러한 확률적 물성치를 반강접 프레임의 비선형 거동에 적용할 수 있는 해석 프로그램을 개발하였다. 프레임의 비선형 거동 인수인 기하학적 비선형, 재료적 비선형, 그리고 접합부의 반강접에 의한 비선형 효과를 고려하여, Monte Carlo Simulation에 기반한 확률론적 해석을 수행하였다. 확률론적 해석을 위해 확률변수를 세 종류로 생성하여 사용하였다. 확정론적 해석의 결과는 기존의 연구 결과와 잘 일치하는 결과를 보였다. 확률론적 해석의 경우, 변위의 분산계수는 구조에 작용하는 하중이 증가함에 따라 증가하는 결과를 나타냈으며, 그 값은 프레임구조의 구조적 특성에 영향을 받는 것으로 나타났다.

Methoxychlor (MXC) was developed to be a replacement for the banned pesticide DDT. HPTE [2,2-bis (p-hydroxyphenyl) -1,1,1-trichloroethane], which is an in vivo metabolite of MXC, has strong oestrogenic and anti-androgenic effects. MXC and HPTE are thought to produce potentially adverse effects by acting through oestrogen and androgen receptors. Of the two, HPTE binds to sex-steroid receptors with greater affinity, and it inhibits testosterone biosynthesis in Leydig cells by inhibiting cholesterol side-chain cleavage enzyme activity and cholesterol utilisation. In a previous study, MXC was shown to induce Leydig cell apoptosis by decreasing testosterone concentrations. I focused on the effects of MXC on male mice that resulted from interactions with sex-steroid hormone receptors. Sexsteroid hormones affect other organs including the kidney and liver. Accordingly, I hypothesised that MXC can act through sex-steroid receptors to produce adverse effects on the testis, kidney and liver, and I designed our experiments to confirm the different effects of MXC exposure on the male reproductive system, kidney and liver. In these experiments, I used pre-pubescent ICR mice; the puberty period in ICR mice is from postnatal day (PND) 45 to PND60. I treated the experimental group with 0, 100, 200, 400 mg MXC/kg b.w. delivered by an intra-peritoneal injection with sesame oil used as vehicle for 4 weeks. At the end of the experiment, the mice were sacrificed under anaesthesia. The testes and accessory reproductive organs were collected, weighed and prepared for histological investigation. I performed a chemiluminescence immune assay to observe the serum levels of testosterone, LH and FSH. Blood biochemical determination was also performed to check for other effects. There were no significant differences in our histological observations or relative organ weights. Serum testosterone levels were decreased in a dose-dependent manner; a greater dose resulted in the production of less testosterone. Compared to the control group, testosterone concentrations differed in the 200 and 400 mg/kg dosage groups. In conclusion, I observed markedly negative effects of MXC exposure on testosterone concentrations in pre-pubescent male mice. From our biochemical determinations, I observed some changes that indicate renal and hepatic failure. Together, these data suggest that MXC produces adverse effects on the reproductive system, kidney and liver.

Mullerian inhibiting substance (MIS) is a member of the TGF-β (transforming growth factor-β) family whose members play key roles in development, suppression of tumour growth, and feedback control of the pituitary-gonadal hormone axis. MIS is expressed in a highly tissue-specific manner in which it is restricted to male Sertoli cells and female granulose cells. The serum levels of MIS in prenatal and postnatal ICR mice were measured using the enzymelinked immuno-solvent assay (ELISA) using the MIS/AMH antibody. Mice were grouped by age: the significant periods were at the onset of development. During sex organ differentiation, no remarkable difference between female and male foetus MIS serum levels (both<0.1 ng/ml) was observed. However, MIS serum levels in pregnant mice markedly changed (4.5～12.2 ng/ml). After birth, postnatal female and male mice serum MIS levels changed considerably (male: <0.1～138.5 ng/ml, female: 5.3～103.4 ng/ml), and the changing phase were diametrically opposed (male: decreasing, female: fluctuating). These findings suggest that MIS may have strong associations with not only develop- ment but also puberty. For further studies, establishing the standard MIS serum levels is of importance. Our study provides the basic information for the study of MIS interactions with reproductive organ disability, cancer, and the effect of other hormone or menopause. We hypothesise that if MIS is regularly injected into middle-age women, meno- pause will be delayed. We detected that serum MIS concentration curves change with age. The changing phase is different between males and females, and this difference is significant after birth. Moreover, MIS mRNA is expressed during the developmental period (prenatal) and also in the postnatal period. This finding indicates that MIS may play a significant role in the developmental stage and in growth after birth.

In this study, we used flow a cytometric assay to evaluate plasma membrane integrity and mitochondrial activity in post-thawed sperm that was supplemented with ginsenoside-Rg1. Varying concentrations of ginsenoside-Rg1 (0, 25, 50 and 100 μM/ml) were used in the extender during cryopreservation to protect the DNA of thawed sperm, thereby increasing the viability and motility rate as evaluated using a computer-assisted sperm analysis (CASA) method. The results derived from CASA were used to compare the fresh, control, and ginsenoside-Rg1 groups. Sperm motility and the number of progressively motile sperm were significantly (p<0.05) higher in the 50 μM/ml ginsenoside-Rg1 group (61.0±4.65%) than in the control (46.6±7.02%), 25 μM/ml (46.2±4.76%), and 100 μM/ml ginsenoside-Rg1 (52.0± 1.90%) groups. However, the velocity distribution of post-thawed sperm did not differ significantly. Membrane integrity and MMP staining as revealed using flow cytometry were significantly (p<0.05) higher (91.6±0.82%) in the 50 μM/ml ginsenoside-Rg1 group than in the other groups. Here, we report that ginsenoside-Rg1 affects the motility and viability of boar spermatozoa. Moreover, ginsenoside- Rg1 can be used as a protective additive for the suppression of intracellular mitochondrial oxidative stress caused by cryopreservation.

The purpose of this study was to analyze and compare the effect of resistance exercise and balance exercise on proprioception and WOMAC index of patients with degenerative knee osteoarthritis. A total of 40 subjects participated in this study. The subjects were diagnosed with degenerative knee osteoarthritis and all were more than 60 years old. They were divided into three groups. Group Ⅰ(n=8) was trained with resistance exercise, Group Ⅱ(n=6) was trained with balance exercise and GroupⅢ(n=6) was trained with range of motion as a control. The results of this study were as follows. It was significantly indicated that the resistance exercise group and balance exercise group elicited error-reduction on proprioception goal-angle (p<.05). There was a statistically significant difference on proprioception between resistance exercise group and control(range of motion) group. There was a statistically significant reduction on WOMAC index between resistance exercise group and balance exercise group (p<.05) and on the WOMAC index between resistance exercise group and range of motion group(p<.05). In conclusion, resistance exercise and balance exercise are effective on degenerative knee osteoarthritis and resistance exercise is the most effective for improving proprioception and WOMAC index. More research on the intervention according to the degree of degenerative knee osteoarthritis is needed.

Interlellkin • 8(IL-8) is an important cytokine involved in tllmor growth and angiogenesis in a variety of malig nancies. bllt the regll lation of IL-8 in 01 외 cancer cells are llnderstood . We invesLigated whether mi togen-activated protein kinases pathway is involved in iron chelator-mediated lL-8 produdion in inunortalized and malignant oral keratinocytes. In this study we examined the role of p38 and extracellular signal- reglllated kinase• 1/2 in the expression of [L-8 by DFO. Incllbation of IHOK and HN12 cel ls with DF'O increased the expression of 11-8 mRNA. as well as the release of IL-8 protein. The signal transdllction study revealed that both p38 and ERK1/2 were significantly activated in response to DFO. Accord ingly. the selective inhibitors for both kinases‘ eit her a lone or combination. abolished DFO- induced lL-8 secretion. indicating an importance of MAP kinase pathway. Interestingly. however‘ inhibition of the p38 and ERK pathway more attenuated IL-8 secretion in IHOK than in HN12 cells. DFO induced NF-kB activation , suggesting a NF-kB- dependent mechanism in DFO- induced IL-8 production. In addition, p38 and ERK inhi bition resulted in the accelerated degradation of lL-8 mRNA, suggesting that in IHOK and HN12 cells, p38 and ERK cunLr iullLe Lo DFO imluced IL-8 secretion by IHOK and HN12 cells via a posttranscriptional mechanism that involves stabilization 01' the IL-8 transcript. Finally. we investigatecl llsing specific inhibitors : 8NP and G8NO for NO c1onor. PDTC for potent inhibitor of NF-kB. Cycloheximide for inhibition of de novo protein synthesis. We observecl 8NP ancl PD1'C clepenclent IL-8 gene incluction in IHOK cell s. but not in HN12 cells used specific inhibitors‘ Collectively. these results demonstrate that‘ targeting MAP kinase ancl NF-kB pathway may be a potentiaI approacb to controlling the angiogenes is ancl growth 이 human oral cancers

목 적 : ORB-ScanII (Version 3 .1 2) 와 DGH500 Ultrasonic pachymetry를 이용하 여 시력교정수술 전 각막중심두께를 측정하여 비교함으로써 수술의 안정성과 정확성 을 높이고자 하였다. 대상과 방법 : 본원에 시력교정수술을 받기 위해 내원한 환자 66 명 130안을 대상 으로 각막두께를 측정하였다. ORB-ScanII 은 어두운 조명에서 3 회를 측정하였고 DGH500 Ultrasonic pachymetry는 20 회 이상 측정하여 중심부의 가장 앓은 각막의 두께를 사용하였다. 두 측정장치의 평균과 표준편차를 알아보았고 일치성은 상관분석 과 회귀분석을 통해 알아보았다. 결 과 : 평균 각막중심두께는 ORB-Scan II z로 측정한 경우는 529 .45 :t 33.34μm 이 였고， DGH500 Ultrasonic pachymetry로 측정한 경우는 525.22 :t 3 1.21μm 이였으며， ORB-ScanII 이 DGH500 Ultrasonic pachymetry보다 평균 4.23 :t 7.91 따l 더 높게 측정 되었다. ORB-ScanII 와 DGH500 Ultrasonic pachymetry 의 상관계수는 0.972로 1 에 가까웠 으며 선형회귀분석을 통하여 회귀식을 구하였다. 결 론 : 두 기기로 측정한 값에서 가장 앓은 각막두께를 기준으로 함으로써 수술 의 안정성을 높였으며 수술 후에 발생할 수도 있는 각막 변형을 예방하는데 도움을 주였다. 하지만， 측정 방식에 따른 측정 장치의 오차를 줄일 수 있는 기술적 발전이 펼요하리라 생각된다.