Large amounts of waste and wastewater from aquaculture have negatively impacted ecosystems. Among them, shrimp aquaculture wastewater contains large amounts of nitrogen contaminants derived from feed residues in an aerobic environment. This study isolated candidate strains from adult rockworms to treat shrimp aquaculture wastewater (SAW) in an aerobic environment. Among 87 strains isolated, 25 grew well at the same temperature as the shrimp aquaculture with excellent polymer degradation ability (>0.5 cm clear zone). Six isolates (strains AL1, AL4, AL5, AL6, LA10, and PR15) were finally selected after combining strains with excellent polymer degradation ability without antagonism. 16S rRNA sequencing analysis revealed that strains AL1, AL4, AL5, AL6, LA10, and PR15 were closely related to Bacillus paramycoides, Bacillus pumilus, Stenotrophomonas rhizophila, Bacillus paranthracis, Bacillus paranthracis, and Micrococcus luteus, respectively. When these six isolates were applied to SAW, they reached a maximum cell viability of 2.06×105 CFU mL-1. Their chemical oxygen demand (CODCr) and total nitrogen (TN) removal rates for 12 h were 51.0% and 44.6%, respectively, when the CODCr/TN ratio was approximately 10.0. Considering these removal rates achieved in this study under batch conditions, these six isolates could be used for aerobic denitrification. Consequently, these six isolates from rockworms are good candidates that can be applied to the field of aquaculture wastewater treatment.

대형저서동물에서 우점하고 있는 갯지렁이류는 퇴적층의 유기물 섭취를 통하여 저질환경개선 에 중요한 역할을 한다. 본 연구에서는 최근 양식기술이 개발된 바위털갯지렁이를 이용하여 갯 벌 퇴적층의 유기물 제거효과를 검토하였다. 이를 위하여 입자특성이 다른 세종류의 저질환경 (S1: 강사질, S2: 해사질, S3: 니사질)에서 해수 및 퇴적층의 TOC를 측정하였다. 바위털갯지렁이 는 세종류의 입자특성에서 모두 유기물 제거효과를 나타내었으며, 입자가 클수록 제거율이 높 았다. 유기물 정화능력을 정량적으로 평가한 결과 바위털갯지렁이는 각 저질별로 강사질에서 3.9856 ppm g-1d-1, 해사질에서 2.8021 ppm g-1d-1, 그리고 니사질에서 28.1142 ppm g-1d-1의 제거능력을 나타내었다. 본 연구결과는 바위털갯지렁이가 다양한 저질에서 유기물 제거에 기 여하고 있음을 보여 주었다.